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Plasmid#203749PurposeEncodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Prolines P8 and P17 in the LAT transmembrane domain mutated to L. Used as a fluorescent plasma membrane probe.DepositorInserttrLATm1 (PtoL) (LAT Human)
UseTagsmEos3.2ExpressionMammalianMutationP8L, P17LPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trLATm2allL+A
Plasmid#203750PurposeEncodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Residues within the transmembrane domain mutated to L and A to increase the TMD interfacial surface area with the membrane. Used as a fluorescent plasma membrane probe.DepositorInserttrLATm2(allL+A) (LAT Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
p249_LTJ_1kbFoxp3wtTemplate
Plasmid#82664Purpose1kb HDR template for murine wildtype Foxp3DepositorInsert1kb genomic DNA sequence from murine wildtype Foxp3
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
p243_LTJ_4kbCD45.1Template
Plasmid#82663Purpose4kb HDR template for murine CD45.1DepositorInsert4kb genomic DNA sequence from murine CD45.1
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p250_LTJ_2kbFoxp3wtTemplate
Plasmid#82665Purpose2kb HDR template for murine wildtype Foxp3DepositorInsert2kb genomic DNA sequence from murine wildtype Foxp3
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPlatTET-gRNA2
Plasmid#82559PurposeAll in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.DepositorInsertdCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
CeNL-Clathrin_pEGFP
Plasmid#89540PurposeCyan color variant of bright luminescent protein, fused to clathrin light chainDepositorInsertCeNL
UseTagsClathrin light chainExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-hMALAT1
Plasmid#118580Purposeto overexpress human MALAT1 in mammalian cellsDepositorInsertMALAT1 (MALAT1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterMSCVAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pF-N-HiBiT-template
Plasmid#200882Purposetemplate for N-terminal HiBIT-taggingDepositorInsertHiBIT-linker
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP703-clathrin
Plasmid#136563PurposeClathrin labeling with a near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)DepositorInsertemiRFP703-clathrin
UseTagsemiRFP703ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEos4a-Clathrin-15
Plasmid#57491PurposeLocalization: Clathrin Vesicles, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertClathrin (CLTB Human)
UseTagsmEos4aExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
T7_CC_Vpx-SIV_WPRE_IVT_Template
Plasmid#216792PurposeTemplate for in vitro transcription of Vpx (SIV).DepositorInsertVpx (SIV)
UseTagsExpressionMammalianMutationPromoterT7Available sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
BB3cH_pGAP_23*_pLAT1_Cas9
Plasmid#104907PurposehCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on HygDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-LATS2
Plasmid#172987Purposeconstitutive expression of FLAG-tagged LATS2DepositorInsert3xFLAG-LATS2 (LATS2 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Clathrin-mGreenLantern
Plasmid#164462PurposeLocalization of mGreenLantern fluorescent protein to clathrinDepositorInsertmGreenLantern
UseTagsClathrinExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-LATS1
Plasmid#172986Purposeconstitutive expression of FLAG-tagged LATS1DepositorInsert3xFLAG-LATS1 (LATS1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-LATS1
Plasmid#23665DepositorInsertLATS1 (LATS1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-bFOSDelataZipVC155
Plasmid#22014DepositorInsertbFosDelataZIPVC155
UseTagsHAExpressionMammalianMutationdeletion of residues 179-193 in bFos(118-211)PromoterAvailable sinceSept. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pClathrin-miRFP670nano3
Plasmid#184667PurposeClathrin labelled with near-infrared fluorescent protein miRFP670nano3DepositorInsertmiRFP670nano3-clathrin light chain
UseTagsmiRFP670nano3ExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc-LATS1
Plasmid#66851PurposeExpress Myc-tagged LATS1 in mammalian cellsDepositorInsertLATS1 (LATS1 Human)
UseTagsMycExpressionMammalianMutationP44L mutation in LATS1 compared to GenBank refere…PromoterCMVAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only