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pGK415-rtetTAK8N, L131L
Plasmid#165969PurposeExpresses rTetTA (K8N, L131L) mutantDepositorInserttetR
UseSynthetic BiologyTagsthree copies of VP16 (VP48)ExpressionYeastMutationPromoterpPGK1Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pATP403red-hENT1
Plasmid#166027PurposeExpresses human equilibrative nucleoside transporter 1 (hENT1)DepositorInserthuman equilibrative nucleoside transporter 1 (SLC29A1 Human)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterpTDH3Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pATP416-pluxO5-ptetO7-crtYBI
Plasmid#165975PurposeExpresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTADepositorInsertsXanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
BTS1 (BTS1 Budding Yeast)
UseSynthetic BiologyTagsExpressionYeastMutationC1281A, G1698A and C66A, C1359TPromoterSynthetic promoter (pluxO5), Synthetic promoter (…Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK415-phlTA2-1E
Plasmid#165970PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pREMI-ZA
Plasmid#183736PurposePlasmid for a restriction enzyme mediated integration (REMI) in Komagataella phaffiiDepositorInsertSh ble
UseRandom insertional gene deletion in komagataella …TagsExpressionMutationPromoterTEF1 promoterAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPAP_scFv
Plasmid#183733PurposeExpresses anti-lysozyme scFv antibodyDepositorInsertAnti-lysozyme scFv
UseTagsHis6-tagExpressionYeastMutationPromoterAOX1 promoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPAP_scFv_V50A
Plasmid#183734PurposeExpresses anti-lysozyme scFv antibody with V50A mutation on MFalpha signalDepositorInsertAnti-lysozyme scFv
UseTagsHis6-tagExpressionYeastMutationV50A mutation in MFalpha signalPromoterAOX1 promoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-hSNCA (A30P)
Plasmid#185720PurposeAAV expression of GFP and human α-Synuclein with A30P mutation from hSyn promoterDepositorInsertsynuclein, alpha (SNCA Human)
UseAAVTagsExpressionMutationChanged Ala 30 to ProPromoterhSynAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
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FUGW-UBC-EGFP-2A-dnVAMP2
Plasmid#185723PurposeLentiviral expression of GFP and dominant-negative VAMP2 from UbC promoterDepositorInsertVAMP-2 (VAMP2 Human)
UseLentiviralTagsExpressionMammalianMutationdeleted AA 97-116PromoterhUbCAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-hSNCA (S129D)
Plasmid#185719PurposeAAV expression of GFP and human α-Synuclein with S129D mutation from hSyn promoterDepositorInsertsynuclein, alpha (SNCA Human)
UseAAVTagsExpressionMutationChanged Ser 129 to AspPromoterhSynAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-hSNCA (S129A)
Plasmid#185718PurposeAAV expression of GFP and human α-Synuclein with S129A mutation from hSyn promoterDepositorInsertsynuclein, alpha (SNCA Human)
UseAAVTagsExpressionMutationChanged Ser 129 to AlaPromoterhSynAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P)
Plasmid#185717PurposeAAV expression of GFP and human α-Synuclein with A11P and V70P mutations from hSyn promoterDepositorInsertsynuclein, alpha (SNCA Human)
UseAAVTagsExpressionMutationChanged Ala 11 to Pro, Val 70 to ProPromoterhSynAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only
