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Showing: 41 - 60 of 2436 results

  1. L0I87-SPCHPromoter_GG0-1

    Plasmid
    #219707
    Purpose
    Level 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pSPCH-GG1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    WT promoter with added golden gate linkers
    Promoter
    Available since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219707-plasmid-map-sequence-id-435861

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  2. L0I84 - GG0-pAHP6-GG1

    Plasmid
    #219706
    Purpose
    Level 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pAHP6-GG1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    WT promoter with added golden gate linkers
    Promoter
    Available since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219706-plasmid-map-sequence-id-435736

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  3. pTALE64G

    Plasmid
    #35188
    Purpose
    Golden Gate Compatible TALE Transcription Factor using VP64 Domain with T2A-GFP addition
    Depositor
    Gang Bao
    Insert
    TAL Effector-VP64-T2A-eGFP
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    +63 C Terminus, VP64 Domain, T2A-GFP
    Promoter
    CMV
    Available since
    Dec. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    35188-plasmid-map-sequence-id-42839

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  4. pTALE64

    Plasmid
    #35189
    Purpose
    Golden Gate Compatible TALE Transcription Factor using VP64 Domain
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Transcription Factor
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    +63 C Terminus, VP64 Domain
    Promoter
    CMV
    Available since
    Dec. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    35189-plasmid-map-sequence-id-68899

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  5. pOGG010

    Plasmid
    #113984
    Purpose
    Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    RK2 (IncP)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113984-plasmid-map-sequence-id-223395

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  6. pOGG011

    Plasmid
    #113985
    Purpose
    Vector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectors
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    pBBR1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113985-plasmid-map-sequence-id-223389

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  7. pOGG012

    Plasmid
    #113986
    Purpose
    Vector construction module. Partition genes (parABCDE) for plasmid stability in absence of antibiotic selection for construction of Golden Gate cloning vectors
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Partition genes (parABCDE)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113986-plasmid-map-sequence-id-223365

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  8. Golden GATEway Cloning Kit

    Plasmid Kit
    #1000000054
    Purpose
    Combinatorial assembly method that combines Golden Gate (Type IIs endonucleases) cloning with Multisite Gateway (recombination) cloning to construct complex plasmids in an efficient and modular way
    Depositor
    Joachim Wittbrodt
    Available since
    Sept. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pOPARA2

    Plasmid
    #200417
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (spectinomycin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200417-plasmid-map-sequence-id-394144

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  10. TALEN_C63

    Plasmid
    #40788
    Purpose
    Golden Gate Compatible TALEN Construct for site specific modification of genome, +63 C Terminus, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    CMV
    Available since
    Dec. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    40788-plasmid-map-sequence-id-68904

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  11. pOPARA1

    Plasmid
    #200416
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200416-plasmid-map-sequence-id-394143

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  12. pCVL RipTAL(Nd154C63) SFFV Xba-Sal linker

    Plasmid
    #50627
    Purpose
    pRipTAL plasmid compatible with Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50627-plasmid-map-sequence-id-79594

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  13. TALEN_C18

    Plasmid
    #47974
    Purpose
    Golden Gate Compatible TALEN Construct site specific modification of genome, shortened C Terminus, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Shortened C-Terminus,Codon Optimized FokI Endonuc…
    Promoter
    CMV
    Available since
    Dec. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    47974-plasmid-map-sequence-id-68905

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  15. Yarrowia lipolytica Golden Gate tool kit

    Plasmid Kit
    #1000000167
    Purpose
    Yarrowia lipolytica Golden Gate tool kit for integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica.
    Depositor
    Tristan Rossignol
    Available since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only

  16. pET-HIS-Sangamo

    Plasmid
    #40786
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    N Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available since
    Dec. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    40786-plasmid-map-sequence-id-68901

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  17. pET-Sangamo-HIS

    Plasmid
    #40787
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    C Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus,Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available since
    Dec. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    40787-plasmid-map-sequence-id-68902

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  18. Golden Gate TALEN and TAL effector kit 2.0

    Plasmid Kit
    #1000000024
    Purpose
    Used to efficiently assemble TALEN constructs with custom repeat arrays, containing anywhere between 12 and 31 repeats. Uses Golden Gate cloning method and has been validated in multiple organisms.
    Depositor
    Adam Bogdanove, Daniel Voytas
    Available since
    Jan. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 41 - 60 of 2436 results