Addgene

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Septin 11 isoform III

Plasmid

#120319

Purpose

Septin 11 isoform III mammalian expression

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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TMEM230-WT-pEGFP(isoform 1)

Plasmid

#99561

Purpose

mammalian expression of TMEM230-GFP (isoform 1)

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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MO70-hCRACR2A-isoform-c

Plasmid

#79591

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Nat Cell Biol. 2010 May;12(5):436-46. do

Insert

CRACR2A-isoform-c (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
TMEM230-WT-IRES(isoform 2)

Plasmid

#99564

Purpose

mammalian expression of TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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Septin 11 isoform II-EGFP

Plasmid

#120316

Purpose

Septin 11 isoform II mammalian expression (EGFP-tag at the C-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pmSin3BSF274 (short form)

Plasmid

#22653

Depositor

Lynn Hudson

Article

Romm et al J Neurochem. 2005 Jun . 93(6):1444-53.

Insert

mSin3B (Sin3b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

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TMEM230-WT-IRES(isoform 1)

Plasmid

#99558

Purpose

mammalian expression of TMEM230 (isoform 1) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMVTRE3G_LYSET-Isoform2_untagged-Puro

Plasmid

#192002

Purpose

Lentiviral vector to generate LYSET(TMEM251)-isoform2 stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform2 (LYSET Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMVTRE3G

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_CMVTRE3G_LYSET-Isoform1_I142V-Puro

Plasmid

#192004

Purpose

Lentiviral vector to generate LYSET(TMEM251)-isoform1 I142V mutant stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform1 I142V (LYSET Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMVTRE3G

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMVTRE3G_LYSET-Isoform1_R45W-Puro

Plasmid

#192003

Purpose

Lentiviral vector to generate LYSET(TMEM251)-isoform1 R45W mutant stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform1 R45W (LYSET Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMVTRE3G

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Septin 11 isoform II

Plasmid

#120318

Purpose

Septin 11 isoform II expression (mCherry-tag at the N-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pBS FGF3 isoform 1

Plasmid

#19230

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Fibroblast growth factor receptor 3 isoform 1 (FGFR3 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
MoClo Plant Parts III: Transformation & Genome Engineering Kit

Plasmid Kit

#1000000236

Purpose

The MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.

Depositor

Daniel Voytas

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only
alpha catenin conformation sensor

Plasmid

#71709

Purpose

This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

Depositor

Deborah Leckband

Article

Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

Insert

alpha-catenin FRET Sensor

Use

Tags

ECFP and YPET

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Basigin-isoform2-CD4d3+4-bio

Plasmid

#73129

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Basigin-isoform2 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Basigin isoform 2 (BSG Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pclbw-opa1(isoform 1)-myc

Plasmid

#62845

Purpose

Mammalian Expression of myc-tagged Opa1 isoform1

Depositor

David Chan

Article

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mmOPA1isoform1-myc (Opa1 Mouse)

Use

Retroviral

Tags

9xMyc

Expression

Mammalian

Mutation

Promoter

cmv-bactin

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3puro-EGFP-FXR1-isoform b

Plasmid

#225449

Purpose

Express EGFP-fusion protein of FXR1 isoform b

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform b (FXR1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MO70-N-Flag-hCRACR2A-isoform-a

Plasmid

#79592

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
CR1-isoformB-CD4d3+4-bio

Plasmid

#73099

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding CR1-isoformB with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

CR1 isoform B (CR1 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-FLAG-Sestrin1 isoform1

Plasmid

#72594

Purpose

overexpression, codon optimized for mammalian expression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN1 (SESN1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only