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Plasmid#79258PurposeExpresses EGFP-tagged DmeIF4E in S2 cellsDepositorInserteIF4E (eIF4E1 Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOCC102-MBP-eIF4A
Plasmid#219981PurposeeIF4A protein purificationDepositorInserteIF4A (TIF1 Budding Yeast)
UseTags6xHis-MBPExpressionInsectMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOCC102-MBP-eIF4G-Sk
Plasmid#219976PurposeeIF4G-Sk protein purificationDepositorInserteIF4G-Sk
UseTags6xHis-MBPExpressionInsectMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOCC22-MBP-eIF4E
Plasmid#219978PurposeeIF4E protein purificationDepositorInserteIF4E (CDC33 Budding Yeast)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_EIF4A2:1-407
Plasmid#211092PurposeMammalian expression of full-length human EIF4A2. C-terminal NanoLuc luciferase tag.DepositorInsertEIF4A2:M1-I407 (EIF4A2 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-eIF4E-TwStrep
Plasmid#208638Purposefor E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)DepositorInserthuman eIF4E (ENST00000450253) (EIF4E Human)
UseTags6xHis and Twin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4E_E
Plasmid#146188PurposeInsect Expression of DmeIF4EDepositorInsertDmeIF4E
UseTagsExpressionInsectMutation3 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-HseIF4AI_T
Plasmid#147542PurposeInsect Expression of HseIF4AIDepositorInsertHseIF4AI (EIF4A1 Human)
UseTagsExpressionInsectMutation3 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-HseIF4AI_T
Plasmid#147543PurposeInsect Expression of HseIF4AIDepositorInsertHseIF4AI (EIF4A1 Human)
UseTagsExpressionInsectMutation3 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOAD eIF4G1 (1-341)
Plasmid#196833PurposeExpesses S.cerevisiae eIF4G1 amino acids 1-341 as Gal4 activation domain fusion.DepositorInserteIF4G1 (TIF4631 Budding Yeast)
UseTagsGal4ExpressionYeastMutationamino acids 1-341PromoterADHAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4A_G
Plasmid#146361PurposeInsect Expression of DmeIF4ADepositorInsertDmeIF4A (eIF-1A Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEK-NvH-DmeIF4E_Z
Plasmid#148195PurposeBacterial Expression of DmeIF4EDepositorInsertDmeIF4E
UseTagsExpressionBacterialMutation3 silent mutations compared to the sequence given…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEK-NvSHN-DmeIF4E_Z
Plasmid#148198PurposeBacterial Expression of DmeIF4EDepositorInsertDmeIF4E
UseTagsExpressionBacterialMutation3 silent mutations compared to the sequence given…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b-eIF4E-S200C
Plasmid#37229DepositorInserteIF4E
UseTagsExpressionBacterialMutationS200CPromoterT7Available sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTYB2-eIF4B-A130C
Plasmid#37227DepositorInsertTIF3
UseTagsExpressionBacterialMutationA130CPromoterT7Available sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ eiF4A1 myc HIS
Plasmid#71657Purposemammalian expression of eiF4A1DepositorInserteiF4A1 (EIF4A1 Human)
UseTagsHIS and mycExpressionMammalianMutationaa403 from Ala to ArgPromoterCMVAvailable sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4G-V5His6
Plasmid#79259PurposeExpresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cellsDepositorInsertDmeIF4G (eIF4G1 Fly)
UseSchneider 2 cell expressionTagsV5-His6 and lambdaN-HAExpressionMutationPromoterAc5 promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ eiF4A2 myc HIS
Plasmid#71658Purposemammalian expression of eiF4A2DepositorInsertEIF4A2 (EIF4A2 Human)
UseTagsHIS and mycExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-GST-eIF4E K119A
Plasmid#112818PurposeExpresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cellsDepositorInsertEif4e (Eif4e Mouse)
UseTagsGSTExpressionBacterialMutationK119 mutated to A, increasing affinity for cap st…PromoterT7Available sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 CMV 3xFG eIF4G1
Plasmid#158775PurposepcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.DepositorInserteIF4G1
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only