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We narrowed to 9,652 results for: crispr plasmids

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TU#1806_CRISPR_unc-73_exon21

Plasmid

#82360

Purpose

to create unc-73E null allele

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Cas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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TU#1805_CRISPR_unc-73_exon2

Plasmid

#82359

Purpose

to create unc-73B null allele

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Cas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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PB-CRISPR

Plasmid

#160047

Purpose

For cloning sgRNA to knockout a specific gene in piggybac plasmid

Depositor

Xiaojun Lian

Article

Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

Type

Empty backbone

Use

CRISPR; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSEVA231-CRISPR

Plasmid

#138712

Purpose

Plasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR Array

Depositor

Víctor de Lorenzo

Article

Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pIW447-CRISPR XYL2

Plasmid

#98906

Purpose

K. marxianus CRISPR Plasmid for XYL2 editing

Depositor

Ian Wheeldon

Article

Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

Inserts

Codon optimized Cas9
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Tags

SV40

Expression

Yeast

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Thy-1-CRISPR-gRNA#1-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124283

Purpose

sgRNA against human Thy-1

Depositor

Csaba Vizler, Katalin Josvay

Insert

Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
Thy-1-CRISPR-gRNA#3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124285

Purpose

sgRNA against human Thy-1

Depositor

Csaba Vizler, Katalin Josvay

Insert

Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
Thy-1-CRISPR-gRNA#2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124284

Purpose

sgRNA against human Thy-1

Depositor

Csaba Vizler, Katalin Josvay

Insert

Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

Plasmid

#175574

Purpose

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

Plasmid

#175573

Purpose

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC-CRISPR

Plasmid

#100008

Purpose

Expresses Cas9-Nlux and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into genome

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis expression

Tags

Cas9-Nlux

Expression

Mutation

Promoter

Ribi promoter

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-CRISPR

Plasmid

#120369

Purpose

Plasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing library

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Inserts

CRISPR1
CRISPR2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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NFATc3-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124288

Purpose

sgRNA against human NFATc3

Depositor

Csaba Vizler, Katalin Josvay

Insert

NFATc3 nuclear factor of activated T cells (NFATC3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
NFATc4-CRISPR-gRNA#exon5-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124290

Purpose

sgRNA against huiman NFATc4

Depositor

Csaba Vizler, Katalin Josvay

Insert

NFATc4 nuclear factor of activated T cells (NFATC4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
NFATc4-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124289

Purpose

sgRNA against human NFATc4

Depositor

Csaba Vizler, Katalin Josvay

Insert

NFATc4 nuclear factor of activated T cells (NFATC4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
LentiCRISPRv2-mCherry

Plasmid

#99154

Purpose

Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

Depositor

Agata Smogorzewska

Inserts

Cas9
mCherry

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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Col7A1-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124287

Purpose

sgRNA against human Col7A1

Depositor

Csaba Vizler, Katalin Josvay

Insert

Collagen type VII alpha 1 chain (COL7A1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
Col7A1-CRISPR-gRNA#exon2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124286

Purpose

sgRNA against human Col7A1

Depositor

Csaba Vizler, Katalin Josvay

Insert

Collagen type VII alpha 1 chain (COL7A1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-sgCTRL

Plasmid

#209750

Purpose

Lentiviral transfer plasmid to express Cas9 and a control, non-specific gRNA (does not target any human gene)

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

Cas9

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-sgCD20

Plasmid

#209749

Purpose

Lentiviral transfer plasmid to express Cas9 and a gRNA targeting the human CD20 gene, MS4A1.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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