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Plasmid#139322PurposePlasmid expressing a sgRNA to introduce BRCA2 V2102I using base editingDepositorInsertsgRNA to insert BRCA2 V2102I using base editing
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA BRCA2 E2772K
Plasmid#139323PurposePlasmid expressing a sgRNA to introduce BRCA2 E2772K using base editingDepositorInsertsgRNA to insert BRCA2 E2772K using base editing
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA BRCA2 R2842C
Plasmid#139324PurposePlasmid expressing a sgRNA to introduce BRCA2 R2842C using base editingDepositorInsertsgRNA to insert BRCA2 R2842C using base editing
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Rat SERCA2b (pMT2)
Plasmid#75184PurposeMammalian expression of ATP2A2DepositorInsertATP2A2
UseTagsExpressionMammalianMutationPromoteradenovirus major late promoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rabbit SERCA2b (pBSII-SK-)
Plasmid#75186PurposeCloning vector for ATP2A2DepositorInsertATP2A2
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-pprA
Plasmid#87744PurposeIPTG-inducible expression of ligase-pprA fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and codon optimized pprA (from Deinococcus …ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-hPMCA2x/b delta6
Plasmid#47587DepositorInsertPMCA2x/b delta6 (ATP2B2 Human)
UseTagsEGFP-HA-HisExpressionMammalianMutation6 C-terminal AA deleted (needed for PDZ binding)PromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgBrca2#2/Cre
Plasmid#173628PurposeExpresses a Brca2-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Brca2 (Brca2 Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
sgRNA BRCA2 S3070F
Plasmid#139325PurposePlasmid expressing a sgRNA to introduce BRCA2 S3070F using base editingDepositorInsertsgRNA to insert BRCA2 S3070F using base editing
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA BRCA2 R2896C
Plasmid#139511PurposePlasmid expressing a sgRNA to introduce BRCA2 R2896C using base editingDepositorInsertsgRNA to insert BRCA2 E2896C using base editing
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-mCherry-hPMCA2w/b
Plasmid#111568PurposeExpresses a plasma membrane calcium pump hPMCA2w/bDepositorUseAAVTagsmCherryExpressionMutationPromoterAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-mCherry-hPMCA2w/b
Plasmid#111570PurposeExpresses a plasma membrane calcium pump hPMCA2w/bDepositorInsertPlasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
GeNL(Ca2+)_480/pcDNA3
Plasmid#85205Purposeluminescent reporter for calcium signalingDepositorInsertGeNL(Ca2+)_480
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
GeNL(Ca2+)_520/pcDNA3
Plasmid#85204Purposeluminescent reporter for calcium signalingDepositorInsertGeNL(Ca2+)_520
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
GeNL(Ca2+)_250/pcDNA3
Plasmid#85206Purposeluminescent reporter for calcium signalingDepositorInsertGeNL(Ca2+)_250
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
CeNL(Ca2+)_1.2μ-pcDNA3
Plasmid#111926PurposeCyan color bright luminescent calcium indicator with intermediate affinityDepositorInsertCeNL(Ca2+)_1.2μ
UseTagsExpressionMammalianMutationE67D, E104D, D133E at CaMPromoterCMVAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
OeNL(Ca2+)_18μ-pcDNA3
Plasmid#111928PurposeOrange color bright luminescent calcium indicator with intermediate affinityDepositorInsertOeNL(Ca2+)_18μ
UseTagsExpressionMammalianMutationE67D, E104D, D133E at CaMPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
CeNL(Ca2+)_19μ-pcDNA3
Plasmid#111927PurposeCyan color bright luminescent calcium indicator with intermediate affinityDepositorInsertCeNL(Ca2+)_19μ
UseTagsExpressionMammalianMutationE67D, F92W, E104D, D133E at CaMPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-NLS-cTF
Plasmid#87746PurposeIPTG-inducible expression of ligase-NLS-cTF fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and NLS linker - cTF (chimeric transcriptio…ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
GeNL(Ca2+)_60/pcDNA3
Plasmid#85207Purposeluminescent reporter for calcium signalingDepositorInsertGeNL(Ca2+)_60
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only