-
Plasmid#29726DepositorTypeEmpty backboneUseTagsBiotin - His6 - TEV and mCeruleanExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits only
-
pcDNA3.1 mycBioID
Plasmid#35700PurposeTo fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tagDepositorHas ServiceCloning Grade DNAInsertBirA
UseTagsMycExpressionMammalianMutationR118G (highly promiscuous form)PromotercmvAvailable sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mCherry LIC cloning vector (MBP-mCherry)
Plasmid#29747DepositorTypeEmpty backboneUseTagsBiotin - His6 - MBP -TEV and mCherryExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET MBP GFP LIC cloning vector (MBP-msfGFP)
Plasmid#29750DepositorTypeEmpty backboneUseTagsBiotin - His6 - MBP -TEV and GFPExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mOrange LIC cloning vector (MBP-mOrange)
Plasmid#29748DepositorTypeEmpty backboneUseTagsBiotin - His6 - MBP -TEV and mOrangeExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mCerulean LIC cloning vector (MBP-mCerulean)
Plasmid#29751DepositorTypeEmpty backboneUseTagsBiotin - His6 - MBP -TEV and mCeruleanExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mCitrine LIC cloning vector (MBP-mCitrine)
Plasmid#29749DepositorTypeEmpty backboneUseTagsBiotin - His6 - MBP -TEV and mCitrineExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Bla/GFP
Plasmid#198221PurposeTransposon vector (Addgene #60510) for doxycycline-inducible expression of EGFPDepositorInsertEGFP
UseTransposonTagsExpressionMammalianMutationNonePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET MBP-FlAsH LIC cloning vector (MBP-FlAsH)
Plasmid#30185DepositorTypeEmpty backboneUseTagsBiotin-H6-MBP-TEV recognition and FlAsHExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 MCS-BirA(R118G)-HA
Plasmid#36047PurposeTo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tagDepositorHas ServiceCloning Grade DNAInsertBirA(R118G)-HA
UseTagsHAExpressionMammalianMutationR118G (highly promiscuous form)PromoterAvailable sinceApril 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET GFP domain 11 LIC cloning vector (GFP C-11)
Plasmid#30183DepositorTypeEmpty backboneUseTagsBiotin-H6-TEV recognition and GFP domain 11ExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET mRuby2 LIC cloning vector (H6-mRuby2)
Plasmid#86934PurposepET LIC cloning vector for Biotin-His6-tev-yORF-mRuby2DepositorTypeEmpty backboneUseTagsBiotin, His6, Tev Recognition Sequence, and mRuby…ExpressionBacterialMutationPromoterT7Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET tdTomato LIC cloning vector (H6-tdTomato)
Plasmid#86928PurposepET LIC cloning vector for Biotin-His6-tev-yORF-tdTomatoDepositorTypeEmpty backboneUseTagsBiotin, His6, Tev Recognition Sequence, and tdTom…ExpressionBacterialMutationPromoterT7Available sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET mClover LIC cloning vector (H6-mClover)
Plasmid#86931PurposepET LIC cloning vector for Biotin-His6-tev-yORF-mCloverDepositorTypeEmpty backboneUseTagsBiotin, His6, Tev Recognition Sequence, and mClov…ExpressionBacterialMutationPromoterT7Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-BioID2-MCS
Plasmid#74223PurposeTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsMyc and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
MCS-BioID2-HA
Plasmid#74224PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
mycBioID2-13X Linker-MCS
Plasmid#92308PurposeTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHumanized A. aeolicus BioID2 with R40G and MycExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
mycBioID2-pBABE-puro
Plasmid#80900PurposeTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); a small MCS is present downstream of myc tag and BioID2 tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagshumanized A. aeolicus BioID2 with R40G and mycExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Alive
Plasmid#20860PurposeAlive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.DepositorInsertStreptavidin-Alive
UseTagsHisExpressionBacterialMutationWt genePromoterAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSBbi_RP_MicroID2-HA
Plasmid#188521PurposeEncodes the biotin proximity labeling enzyme MicroID2 for expression and stable integration into mammalian cellsDepositorInsertMicroID2
UseTransposon mediated gene integrationTagsHAExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only