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Plasmid#85165PurposeProvides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.DepositorInsertEcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
UseTagsAmilCP and RTXExpressionBacterialMutationPromoterJ23100 promoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTOX6
Plasmid#127451PurposeEncodes Tse2 toxin and amilCP chromoprotein. CAM-RDepositorInsertstse2 toxin
amilCP chromoprotein
UseTagsExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTOX4
Plasmid#127449PurposeEncodes YhaV toxin and amilCP chromoprotein. CAM-RDepositorInsertsyhaV toxin
amilCP chromoprotein
UseTagsExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTOX5
Plasmid#127450PurposeEncodes MqsR toxin and amilCP chromoprotein. CAM-RDepositorInsertsmqsR toxin
amilCP chromoprotein
UseTagsExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pR562
Plasmid#207886PurposeCreation of Level 0 entry clonesDepositorInsertamilCP
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
PGR-Blue
Plasmid#68374PurposeTerminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.DepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assemblyTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUS250
Plasmid#198322PurposeCumate-inducible gene expression in diverse gram negative bacteria. Features also include mobilisation via oriT, a gigantic multiple cloning site, and blue/white screening via amilCP chromoproteinDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromotercumate-inducibleAvailable sinceApril 12, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
lvl0 MethylAblet
Plasmid#153412PurposeIt is used to bypass the Level 1 cloning and directly feed terminators into Level 2DepositorInsertamilCP
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK1010 (pSL25)
Plasmid#191005PurposeExpresses blue chromoprotein on a broad host range RP4 origin plasmidDepositorInsertblue chromoprotein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterCP25Available sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGA-blue
Plasmid#196336PurposeEasy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screeningDepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assembly backboneTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
mUAV
Plasmid#102680PurposeMobius Assembly Universal Acceptor Vector, it converts amplified PCR fragments into standard, exchangeable partsDepositorInsertJ23106:B0034:amilCP:rrnBT1-T7TE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Chromoprotein kit 2.0
Plasmid Kit#1000000198PurposeChromoprotein plasmid kit that contain two new chromoprotein genes that are less toxic to E. coli so that the plasmids are more stable during growth.DepositorAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Chromoprotein kit 1.0
Plasmid Kit#1000000174PurposeA synthetic biology collection of plasmids for expression of chromoproteins in bacteria, which can be used as an educational resource.DepositorAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only