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Plasmid#20711DepositorInsertItk TEC (Itk Mouse)
UseTagsGSTExpressionBacterialMutationamino acids 97-174PromoterAvailable sinceMarch 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
DTECT - Plasmid for Standard Curve
Plasmid#139333PurposeThis plasmid is used to generate the unique standard curve required to quantify the capture of genomic signature using DTECTDepositorInsertDTECT ligation product
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH773_pTech_PlldR-HHRz-supP(opal)
Plasmid#163732PurposeLactate-inducible expression of supP(opal), preceded by a hammerhead ribozymeDepositorInserthhrz-supP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTH861-gst-optec P210T
Plasmid#89595PurposePlasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase P210T mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged proline 210 to threoninePromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH860-gst-optec A180Q
Plasmid#89594PurposePlasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged alanine 180 to glutaminePromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH858-gst-optec V156E
Plasmid#89592PurposePlasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase V156E mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged valine 156 to glutamic acidPromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH859-gst-optec Y172M
Plasmid#89593PurposePlasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationchanged tyrosine 172 to methioninePromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3-Prpl28-ACDH-Tecm10
Plasmid#127745PurposeYeast pathway position 7. ACDH transcription unit with the RPL28 promoter and ECM10 terminator.DepositorInsertacetylating acetaldehyde dehydrogenase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPrpl28Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag TEC
Plasmid#20649DepositorInsertTEC (TEC Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
Calprotectin S100A9 toehold switch sensor
Plasmid#110717PurposeToehold switch sensor to detect Calprotectin S100A9 mRNA with GFP outputDepositorInsertCalprotectin S100A9 toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Syn:NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEcs(ENLYFQ/M)-tTA
Plasmid#135462PurposeNeuronal expression of uFLARE TF componentDepositorInsertNRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEVcs(ENLYFQ/M)-tTA
UseAAVTagsHA, flag, VP16ExpressionMammalianMutationPromoterSynapsinAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flp-DOG-NW (AAV1)
Viral Prep#75469-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-Flp-DOG-NW (#75469). In addition to the viral particles, you will also receive purified pAAV-EF1a-Flp-DOG-NW plasmid DNA. EF1a-driven expression of Flp recombinase dependent on GFP (Flp-DOG); to be coinjected with GFP (or for use with transgenic GFP animals). These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF-1aTagsNoneAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NE1h (AAV9)
Viral Prep#123309-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-GRAB_NE1h (#123309). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_NE1h plasmid DNA. hSyn-driven expression of GRAB-NE1h norepinephrine sensor in neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NE1m (AAV9)
Viral Prep#123308-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-GRAB_NE1m (#123308). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_NE1m plasmid DNA. Synapsin-driven expression of GRAB-NE1m norepinephrine sensor in neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_OT1.0 (AAV9)
Viral Prep#185386-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-GRAB_OT1.0 (#185386). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_OT1.0 plasmid DNA. hSyn-driven expression of the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_OT1.0 (AAV1)
Viral Prep#185386-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hSyn-GRAB_OT1.0 (#185386). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_OT1.0 plasmid DNA. hSyn-driven expression of the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_OTmut (AAV9)
Viral Prep#185388-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-GRAB_OTmut (#185388). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_OTmut plasmid DNA. hSyn-driven expression of the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_OTmut (AAV1)
Viral Prep#185388-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hSyn-GRAB_OTmut (#185388). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_OTmut plasmid DNA. hSyn-driven expression of the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby (AAV1)
Viral Prep#71760-AAV1PurposeReady-to-use AAV1 particles produced from pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby (#71760). In addition to the viral particles, you will also receive purified pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby plasmid DNA. Syn-driven, Cre-dependent expression of membrane-bound GFP and the presynaptic protein synaptophysin fused to mRuby. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsmGFP, mRuby (Cre-dependent)Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_DA1m (AAV9)
Viral Prep#113049-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-GRAB_DA1m (#113049). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_DA1m plasmid DNA. Synapsin-driven expression of GRAB-DA1m dopamine sensor in neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only