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Plasmid#179668PurposeMembrane-bound (Fyn) TEVP-inducible caged-cTVMVPDepositorInsertCaged cTVMVP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-Lyn-nTVMVpmut-tevs-AP4-tevs-P3-cTVMVp
Plasmid#179672PurposeMembrane-bound (Lyn) TEVP-inducible caged-cTVMVPDepositorInsertCaged cTVMVP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-nTVMVp-AP4-tevs-P3-tevs-cTVMVPmut-CAAX
Plasmid#179662PurposeMembrane-bound (CAAX) TEVP-inducible caged-nTVMVPDepositorInsertCaged-nTVMVP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
V2-MESA-35F-Tev
Plasmid#84503PurposeMESA protease chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, and Tev proteaseDepositorInsertV2-MESA-35F-Tev
UseAAVTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
V1-MESA-45F-Tev
Plasmid#84501PurposeMESA protease chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, and Tev proteaseDepositorInsertV1-MESA-45F-Tev
UseAAVTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
Rap-MESA-20-Tev
Plasmid#84555PurposeMESA protease chain with rapamycin FKBP ectodomain, 20 extracellular linkers, and Tev proteaseDepositorInsertRap-MESA-FKBP-20-Tev
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
His6-MBP-uTEV3
Plasmid#135464PurposeBacterial expression of uTEV3 (full-length) proteaseDepositorInsertHis6-MBP-uTEV3
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation (improves stability) , I138T/S153N…PromoterT5Available sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pREP3-TEV-NLS
Plasmid#21146DepositorInsertTEV protease (TEVgp1 tobacco etch virus)
UseS. pombeTags2xNLS, NLS, and mycExpressionYeastMutationPromoterAvailable sinceJuly 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
CMV:eGFP-CaM-uTEV1Δ(220-242)
Plasmid#135461PurposeMammalian expression of uFLARE protease componentDepositorInserteGFP-CaM-uTEV1Δ
UseTagseGFP, V5ExpressionMammalianMutationS219V mutation improves stability and S153N impro…PromoterCMVAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30b-5M SrtA with TEV site
Plasmid#86962PurposeExpresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tagDepositorInsertS.aureus SrtA 5M
UseTags6x Histidine tag and TEV siteExpressionBacterialMutationP94R, D160N, D165A, K190E, K196TPromoterT7Available sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-puro-2A-TEVp
Plasmid#99610PurposeLentivirus expressing puromycin resistance gene and TEV proteaseDepositorInsertsPuromycin resistance (PAC gene)
TEV protease
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ca-FLARE (protease)
Plasmid#92214Purposeprotease component of FLAREDepositorInsertCaM-V5-TEVp(Δ220-242)
UseAAVTagsExpressionMammalianMutationPromotersynapsinAvailable sinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQC_HA-p115 TEV-Myc #77#407
Plasmid#42127DepositorInsertp115
UseRetroviralTagsHA and MycExpressionMammalianMutationinserted ENLYFQ b/w 886 and 887PromoterCMVAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
Syn:eGFP-CaM-uTEV1Δ(220-242)
Plasmid#135463PurposeNeuronal expression of uFLARE protease componentDepositorInserteGFP-CaM-uTEV1Δ
UseAAVTagseGFP, V5ExpressionMammalianMutationS219V mutation improves stability and S153N impro…PromoterSynapsinAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_HA-p115-TEV-cleaved #957#382
Plasmid#42107DepositorInsertp115
UseTagsHAExpressionMammalianMutationdeleted 886 to end and added ENLYFQPromoterCMVAvailable sinceFeb. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pelBHisTEV
Plasmid#157741Purposeempty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sitesDepositorTypeEmpty backboneUseTagsPelB signal sequence + His10-tag + TEV protease c…ExpressionBacterialMutationPromoterT7lacAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV:eGFP-p2A-HA-β2Ar-uTEV1Δ(220-242)
Plasmid#135459PurposeMammalian expression of SPARK protease componentDepositorInserteGFP-p2A-HA-βarrestin2-uTEV1Δ
UseLentiviralTagseGFP, HAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQC.Flip-nluc(LgBiT1-8)CP[TEV]
Plasmid#200122PurposeRetroviral expression of Flip-nluc(LgBiT1-8)CP[TEV] protease reporter and T2A mCherry in mammalian cellsDepositorInsertFlip-nluc(LgBiT1-8)CP[TEV] T2A mCherry
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-melHis10TEV
Plasmid#159428Purposeempty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sitesDepositorTypeEmpty backboneUseTagsmelittin signal sequence (removed in vivo by sign…ExpressionInsectMutationPromoterpolyhedrinAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TEVp
Plasmid#162599PurposeTEV protease under CMV promoterDepositorInsertTEV protease
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only