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Plasmid#63627PurposeCTGA-R13-AKSIRV RNAP mutantDepositorInsertCTGA-R13-AKSIRV RNAP
UseTagsn/aExpressionMutationV725A, Q744K, L747I, N748S, L749I, R756T, Q758K, …PromoterT5/LacAvailable sinceJune 3, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE-WT-N4-R5-RNRV
Plasmid#63630PurposeN4-R5-RNRV RNAP mutantDepositorInsertN4-R5-RNRV RNAP
UseTagsExpressionMutationL747I, N748D, L749C, M750V, F751I, Q754T, F755R, …PromoterT5/LacAvailable sinceApril 15, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE-WT-N4-R5-YRNRV
Plasmid#63625PurposeN4-R5-YRNRV RNAP mutantDepositorInsertN4-R5-YRNRV RNAP
UseTagsExpressionMutationN671Y, L747I, N748D, L749C, M750V, F751I, Q754T, …PromoterT5/LacAvailable sinceJune 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE-WT-CTGA-R13-AKSRV
Plasmid#63622PurposeCTGA-R13-AKSRV RNAP mutantDepositorInsertCTGA-R13-AKSRV RNAP
UseTagsExpressionBacterialMutationV725A, Q744K, L747I, N748S, R756T, Q758K, H772R, …PromoterT5/LacAvailable sinceJuly 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTHSSd_44
Plasmid#59966Purposeconstitutive expression of T7 sigma fragmentDepositorInsertT7 sigma fragment
UseTagsExpressionBacterialMutationN/APromoterPcon sigmaAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
p6-8
Plasmid#118089Purposerapa-T7- mRNA(GFP)DepositorInsertRapamycin-inducible split T7 RNAP with T7 driven mRNA for GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTSara-C
Plasmid#60722PurposeExpresses the C-terminal fragment of split T7 RNAP split a position 179. Insert is driven by the arabinose inducible promoter PBAD. Contains a constitutive araC ORFDepositorInsertResidues 180-880 of T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationOnly amino acids 180-880 of T7 RNAPPromoterPBADAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSara-N
Plasmid#60721PurposeExpresses the N-terminal fragment of split T7 RNAP split a position 179. Insert is driven by PBAD. Contains a constitutive araC ORFDepositorInsertResidues 1-179 of T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationOnly amino acids 1-179 of T7 RNAPPromoterPBADAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-wt
Plasmid#60728PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
BioBrick pET-24a(+)
Plasmid#117802PurposeBioBrick pET vector for protein overexpression in E. coliDepositorTypeEmpty backboneUseEscherichia coliTagsExpressionMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTSara
Plasmid#60720PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORFDepositorInsertsN Terminal fragment of T7 RNAP split betwen 179-180
N Terminal fragment of T7 RNAP split betwen 179-180
UseTagsExpressionBacterialMutationT7 RNAP split between positions 179-180 and wt T7…PromoterPBADAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-Q758C
Plasmid#60729PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758CDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSara-R756K
Plasmid#60727PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756K. Contains a constitutive araC ORFDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-N748D
Plasmid#60732PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-R756K
Plasmid#60731PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756SDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSara-N748D
Plasmid#60726PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation N748D. Contains a constitutive araC ORFDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-R756S
Plasmid#60730PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756SDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTSara-R756S
Plasmid#60725PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756S. Contains a constitutive araC ORFDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSara-Q758C
Plasmid#60724PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation Q758C. Contains a constitutive araC ORFDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pY71-tet.I.8
Plasmid#133521PurposesfGFP driven by a T7 promoter with the tetO sequence 11 bp downstream from the promoter start siteDepositorInsertsfGFP
UseTagsStrep tag and TEV siteExpressionBacterialMutationWTPromoterT7Available sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only