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Showing: 41 - 60 of 9450 results

  6. GABA(A) receptor subunit g2SE

    Plasmid
    #49170
    Purpose
    pHluorin-tagged GABA A receptor subunit (gamma 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Stephen Moss, Tija Jacob
    Article
    Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.
    Insert
    GABA(A) receptor subunit gamma 2 (Gabrg2 Mouse)
    Use
    Tags
    myc, pHGFP (pHluorin), and thrombin cleavage site…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49170-plasmid-map-sequence-id-165320

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  7. GABA(A) receptor subunit B3SE

    Plasmid
    #49171
    Purpose
    pHluorin-tagged GABA A receptor subunit (beta 3) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Stephen Moss, Tija Jacob
    Article
    Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.
    Insert
    GABA(A) receptor subunit beta 3 (Gabrb3 Mouse)
    Use
    Tags
    myc, pHGFP (pHluorin), and thrombin cleavage site…
    Expression
    Mammalian
    Mutation
    GTG (V) to TGC (C)
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49171-plasmid-map-sequence-id-72502

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  8. GABA(A) receptor subunit a1SE

    Plasmid
    #49168
    Purpose
    pHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Stephen Moss, Tija Jacob
    Article
    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do
    Insert
    GABA(A) receptor subunit alpha-1 (Gabra1 Mouse)
    Use
    Tags
    myc and pHGFP (pHIuorin)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49168-plasmid-map-sequence-id-72484

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  9. GABA(A) receptor subunit a2SE

    Plasmid
    #49169
    Purpose
    pHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Stephen Moss, Tija Jacob
    Article
    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do
    Insert
    GABA(A) receptor subunit alpha-2 (GABRA2 Human)
    Use
    Tags
    HA, bovine alpha 1 signal sequence, and pHGFP (pH…
    Expression
    Mammalian
    Mutation
    T343A
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49169-plasmid-map-sequence-id-72495

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  10. pcDNA3-AMPKsub-YPet-NES

    Plasmid
    #84635
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub-YPet-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    84635-plasmid-map-sequence-id-176207

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  11. pcDNA3-AMPKsub(TA)-YPet-NES

    Plasmid
    #84636
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    AMPKsub(TA)-YPet-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    April 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    84636-plasmid-map-sequence-id-176880

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  13. pcDNA3-PKCsub-YPet-NES

    Plasmid
    #138207
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKCsub-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    138207-plasmid-map-sequence-id-307410

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  14. pIVTR(T7A)-ATOH1 (SA Substitutions)

    Plasmid
    #172309
    Purpose
    In vitro transcription of ATOH1 (Ser phosphosites substituted by Ala)
    Depositor
    Patrik Verstreken
    Insert
    ATOH1 (ATOH1 Human)
    Use
    Other
    Tags
    Expression
    Mutation
    S331A, S342A
    Promoter
    T7 promoter A-initiating
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172309-plasmid-map-sequence-id-340456

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  15. pIVTR(T7A)-NGN2 (SA Substitutions)

    Plasmid
    #172308
    Purpose
    In vitro transcription of NGN2 (Ser phosphosites substituted by Ala)
    Depositor
    Patrik Verstreken
    Insert
    NGN2
    Use
    Other
    Tags
    Expression
    Mutation
    S24A, S193A, S207A, S209A, S219A, S232A, S239A an…
    Promoter
    T7 promoter A-initiating
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172308-plasmid-map-sequence-id-340455

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  16. psubCAG-WPRE-hVEGF-D-Strept

    Plasmid
    #119227
    Purpose
    Plasmid encodes Strep-tagged full-length (unprocessed) VEGF-D of human origin to be used for stimulation of lymphangiogenesis in mice in vivo.
    Depositor
    Kari Alitalo
    Insert
    VEGFD (VEGFD Human)
    Use
    AAV
    Tags
    Strept
    Expression
    Mutation
    Promoter
    CAG
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    119227-plasmid-map-sequence-id-231678

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  17. psubCMV-mVEGF-D-dNdC-WPRE

    Plasmid
    #119228
    Purpose
    Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has native signal peptide to be used for stimulation of lymphangiogenesis in mice in vivo.
    Depositor
    Kari Alitalo
    Insert
    Vegfd-dN-dC (Vegfd Mouse)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    119228-plasmid-map-sequence-id-231686

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  18. pcDNA3-PKAsub-YPet-NES

    Plasmid
    #138203
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub-YPet-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    138203-plasmid-map-sequence-id-307404

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  19. pcDNA3-JNKsubDD-YPet-NES

    Plasmid
    #84633
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    JNKsubDD-YPet-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    84633-plasmid-map-sequence-id-176152

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  20. pcDNA3-JNKsub(TA)DD-YPet-NES

    Plasmid
    #84634
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    JNKsub(TA)DD-YPet-NES
    Use
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    84634-plasmid-map-sequence-id-176198

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Showing: 41 - 60 of 9450 results