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pACEBac1-CoRESTfl
Plasmid#109156PurposeEncodes human full-length CoREST with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length CoREST, sequence-optimized for insect cells (RCOR1 Human)
UseTags3xFLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1REST_sh_seq2_WPRE
Plasmid#127574PurposeshRNA to knock down RE1-silencing transcription factor (REST)DepositorInsertshRNA for knock down of REST (REST Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-REST_sh_seq1_WPRE
Plasmid#127573PurposeshRNA to knock down RE1-silencing transcription factor (REST)DepositorInsertshRNA for knock down of REST (REST Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pREST-donor
Plasmid#113807PurposeCRISPR donor plasmid to tag human transcription factor REST with GFPDepositorInsertREST homology arms flanking EGFP-IRES-Neo cassette (REST Human)
UseCRISPRTagsExpressionMutationrs4864608PromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_PresT-mCherryBb
Plasmid#118238PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes resT promoter driven mCherryDepositorInsertresT promoter driven mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PresTL-sgRNA500
Plasmid#149617PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPresTLAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_PresTS-sgRNAflaB
Plasmid#149644Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PresTSAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_PresTL-sgRNAflaB
Plasmid#149645Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PresTLAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PresTS-sgRNA500
Plasmid#149616PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPresTSAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PresTS-sgRNAflaB
Plasmid#149624PurposeflaB gRNA expression in B. burgdorferiDepositorInsertsgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPresTSAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PresTL-sgRNAflaB
Plasmid#149625PurposeflaB gRNA expression in B. burgdorferiDepositorInsertsgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPresTLAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pACEBAC1-CoREST-C
Plasmid#109159PurposeEncodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)
UseTags3xFLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-CREST (C89)
Plasmid#22961DepositorInsertCREST (Ss18l1 Rat)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLOX-Ttag-iresTK
Plasmid#12246DepositorInsertSV40 Large T, HSV1-TK (SV40gp6 Simian virus)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationLoxP in 3' LTR.PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
beta-arrestin2 mYFP
Plasmid#36917DepositorInsertbeta-arrestin2 (Arrb2 Rat)
UseTagsmYFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
Beta-arrestin2 GFP WT
Plasmid#35411DepositorInsertβarrestin2 (Arrb2 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
beta-arrestin1 mYFP
Plasmid#36916DepositorInsertbeta-arrestin1 (Arrb1 Rat)
UseTagsmYFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLG034 (restriction-like)
Plasmid#157912PurposeExpresses a restriction-like defense systemDepositorInsert4-gene restriction-like system
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only
