Addgene

We narrowed to 76 results for: Plk1

Showing: 41 - 60 of 76 results

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Plk1 FRET sensor c-jun substrate

Plasmid

#45203

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Plk1 FRET sensor with c-jun substrate

Use

Tags

CyPET, YPET

Expression

Mammalian

Mutation

Promoter

T7

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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PLK1-cjun-FRET-PACT biosensor

Plasmid

#106907

Purpose

centrosome-localized PLK1 FRET-activity biosensor (through PACT domain with 10aa linker)

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

PLK1-cjun-FRET-PACT biosensor with linker (PLK1 Human)

Use

Tags

PACT domain

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PLK1

Plasmid

#20589

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PLK1 (PLK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pRcCMV myc-Plk1 T210D (Nigg LA183)

Plasmid

#41158

Depositor

Erich Nigg

Article

Smits et al Nat Cell Biol. 2000 Sep;2(9):672-6.

Insert

Plk1 (PLK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

T210D

Promoter

CMV

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
pRcCMV myc-Plk1 K82R (Nigg LA184)

Plasmid

#41157

Depositor

Erich Nigg

Article

Smits et al Nat Cell Biol. 2000 Sep;2(9):672-6.

Insert

Plk1 (PLK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

K82R

Promoter

CMV

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
untargeted Plk1 FRET sensor Myt1 substrate

Plasmid

#45217

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

untargeted Plk1 FRET sensor Myt1 substrate

Use

Tags

Expression

Mammalian

Mutation

Promoter

t7

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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N-term Hec1 Plk1 FRET sensor c-jun substrate

Plasmid

#45204

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

Insert

Hec1-targeted Plk1 FRET sensor

Use

Tags

CyPET, YPET

Expression

Mammalian

Mutation

Promoter

Available since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 Plk1mut7A-C-PAMmut-Myc-DDK-P2A-Puro

Plasmid

#211616

Purpose

Rhno1 Plk1mut7A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

S51A, T52A, S141A, S162A, S163A, S164A, S178A

Promoter

CMV

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PBD wt (Nigg AH12)

Plasmid

#41162

Depositor

Erich Nigg

Article

Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

Insert

PBD (PLK1 Human)

Use

Tags

3xMyc and PreScission Site

Expression

Mammalian

Mutation

Contains Plk1-PBD only (aa 326–603)

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3 PBD HK-AA (Nigg AH13)

Plasmid

#41161

Depositor

Erich Nigg

Article

Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

Insert

PBD HK-AA (PLK1 Human)

Use

Tags

3xMyc and PreScission Site

Expression

Mammalian

Mutation

Contains Plk1-PBD only (aa 326–603); HK-AA (H538A…

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only
pIC195

Plasmid

#69730

Purpose

Retroviral expression of Polo-Like Kinase 1 (Plk1)

Depositor

Iain Cheeseman

Insert

Polo-Like Kinase 1 (Plk1) (PLK1 Human)

Use

Retroviral

Tags

eYFP-TEV-S

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pKM065

Plasmid

#134175

Purpose

Retroviral plasmid expressing GFP-NLS-Plk1-PBD

Depositor

Iain Cheeseman

Article

McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

Insert

GFP-NLS-Plk1-PBD 326-603 (PLK1 Human)

Use

Retroviral

Tags

eGFP-TEV-S

Expression

Mammalian

Mutation

PBD 326-603

Promoter

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pTK24

Plasmid

#37406

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Plk1 (PLK1 Human)

Use

Tags

Mem-mCherry, S-tag, and TEV

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pTK25

Plasmid

#37407

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Plk1 (PLK1 Human)

Use

Tags

Mem-mCherry, S-tag, and TEV

Expression

Mammalian

Mutation

K82R

Promoter

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP PICH (Nigg CB62)

Plasmid

#41163

Depositor

Erich Nigg

Article

Baumann et al Cell. 2007 Jan 12;128(1):101-14.

Insert

PICH (ERCC6L Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pBABE-SSeCKs

Plasmid

#106908

Purpose

full-length murine SSeCKs/AKAP12/Gravin in retroviral vector

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

SSeCKs (Akap12 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

gag

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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(PD-S608A)FLAG-Cep215-FL

Plasmid

#106905

Purpose

Expresses murine non-phosphorylatable Cep215 at S608

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

CEP215 (Cdk5rap2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine-608 to Alanine

Promoter

CMV

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM328

Plasmid

#134243

Purpose

Mammalian expression construct expressing sgRNA against PLK1

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

gRNA targeting PLK1 3'

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBABE-SSeCKs T751A

Plasmid

#107142

Purpose

full-length murine SSeCKs/AKAP12/Gravin T751A (analogous to T766A mutation in human) in retroviral vector

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

SSeCKs T751A (Akap12 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed threonine 751 to alanine

Promoter

gag

Available since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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(PM-S608E)FLAG-Cep215-FL

Plasmid

#106906

Purpose

Expresses murine phospho-mimetic CEP215 at S608

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

CEP215 (Cdk5rap2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine-608 to Glutamic Acid

Promoter

CMV

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge