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Plasmid#232433PurposepegRNA with optimized 3' modifications to correct the rd6 mutationDepositorInsert3'-PP7-tagged pegRNA for rd6 correction driven by human U6 promoter
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6_rd12_pegRNA_(PP7-C4-Q1)
Plasmid#232435PurposepegRNA with optimized 3' modifications to correct the rd12 mutationDepositorInsert3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6_empty-sgRNA(PP7)
Plasmid#232432Purposeempty gRNA backbone which contains the PP7 aptamer in the scaffold tetraloopDepositorInsertPP7-tagged sgRNA scaffold driven by human U6 promoter
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-tdPP7-3xmCherry
Plasmid#74926PurposeExpresses tdPP7-3xmCherryDepositorInsertPP7-3xmCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETZZtevPP7CP(C68A,C73A)
Plasmid#27548DepositorInsertPseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
UseTagsTEV protease cleavage site, six histidine tag, an…ExpressionBacterialMutationchanged two cysteine to alanine (C68A,C73A) to in…PromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter
Plasmid#61762PurposeDual Color betaGlobin reporter to study splicing kinetics in vivoDepositorInsertb-Globin
UseTags24 X MS2 hairpin cassette in 3'UTR of b-Glob…ExpressionMammalianMutationPromoterDox inducible Tet-ON promoterAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA1-2XPP7
Plasmid#75390PurposesgRNA1-2XPP7DepositorInsertsgRNA1-2XPP7
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
psPAX2-D64V-NC-PP7
Plasmid#122945PurposePP7 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an PP7 aptamer into lentivirus-like particlesDepositorInsertNC-PCP (PP7 coat protein) fusion protein in Gag
UseLentiviralTagsExpressionMammalianMutationD64VPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
cmv-mCherry-10xPP7
Plasmid#185796PurposemCherry labeled with 10xPP7DepositorInsertcmv-mCherry-10xPP7
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
DHFR-PP7-VP64_T2A_GFP
Plasmid#86167PurposeExpresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cellsDepositorInsertDHFR-PP7-VP64_T2A_GFP
UseCRISPR and LentiviralTagsDestabilized domain (N-terminus version) of E.col…ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
cmv-mCherry-8xPP7
Plasmid#185795PurposemCherry labeled with 8xPP7DepositorInsertcmv-mCherry-8xPP7
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDZ617 pKAN 12xPP7 V4
Plasmid#72237PurposeFloxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interestDepositorInsert12xPP7 V4
UseCre/LoxTagsExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pscPP7(2xWT)-EGFP
Plasmid#140261PurposePP7CP-dependent translational repressionDepositorInsertTwo PP7CP-binding sites embedded in scaffold
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
psgRNA2.02-PP7-ccdB
Plasmid#99912PurposeBasic cloning vector for the expression of PP7 inserted sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
sg2.0 (PP7) Locus#2
Plasmid#101155PurposegRNA with two PCP binding sitesDepositorInsertLocus #2(tandem repeat)
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS42K_PCP-VP64_B-PP7
Plasmid#177157PurposeExpresses transactivator (PCP-VP64) and scRNA (B-PP7) in yeast cellsDepositorInsertsPCP-VP64
B-PP7
UseTagsSV40 NLSExpressionYeastMutationPromoterADH1 promoter and SNR52 promoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS42K_PCP-VP64_A-PP7
Plasmid#177156PurposeExpresses transactivator (PCP-VP64) and scRNA (A-PP7) in yeast cellsDepositorInsertsPCP-VP64
A-PP7
UseTagsSV40 NLSExpressionYeastMutationPromoterADH1 promoter and SNR52 promoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
MPP7 gRNA (BRDN0001145242)
Plasmid#75832Purpose3rd generation lentiviral gRNA plasmid targeting human MPP7DepositorInsertMPP7 (MPP7 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MPP7 gRNA (BRDN0001147517)
Plasmid#75833Purpose3rd generation lentiviral gRNA plasmid targeting human MPP7DepositorInsertMPP7 (MPP7 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MPP7 gRNA (BRDN0001149493)
Plasmid#75834Purpose3rd generation lentiviral gRNA plasmid targeting human MPP7DepositorInsertMPP7 (MPP7 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only