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Plasmid Kit#1000000238PurposeThe collection contains a selection of diatom promoters and terminators from the organism Phaeodactylum tricornutum on vectors compatible with the uLoop cloning system.DepositorAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only
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pAAV-Ef1a-DIO-PPO-Venus (AAV9)
Viral Prep#139505-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-Ef1a-DIO-PPO-Venus (#139505). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-PPO-Venus plasmid DNA. EF1a-driven, Cre-dependent expression of Venus-tagged parapinopsin for spatiotemporal control of inhibitory GPCR signaling cascades. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsVenus (Cre-dependent)Available sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-PPO-Venus (AAV8)
Viral Prep#139505-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-Ef1a-DIO-PPO-Venus (#139505). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-PPO-Venus plasmid DNA. EF1a-driven, Cre-dependent expression of Venus-tagged parapinopsin for spatiotemporal control of inhibitory GPCR signaling cascades. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsVenus (Cre-dependent)Available sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-PPO-Venus (AAV5)
Viral Prep#139504-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CAG-DIO-PPO-Venus (#139504). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-PPO-Venus plasmid DNA. CAG-driven, Cre-dependent expression of Venus-tagged parapinopsin. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsVenusAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM9_fcpA_HD
Plasmid#90416PurposeTALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_HDDepositorInsertTALEN Backbone
UsePhaeodactylum tricornutumTagsExpressionMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pM9_NR_HD
Plasmid#90415PurposeTALEN Backbone for Phaeodactylum tricornutum_ NR promotor_HDDepositorInsertTALEN Backbone
UsePhaeodactylum tricornutumTagsExpressionMutationPromoterAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pM9_NR_NG
Plasmid#90423PurposeTALEN Backbone for Phaeodactylum tricornutum_ NR promotor_NGDepositorInsertTALEN Backbone
UsePhaeodactylum tricornutumTagsExpressionMutationPromoterAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pM9_NR_NN
Plasmid#90421PurposeTALEN Backbone for Phaeodactylum tricornutum_ NR promotor_NNDepositorInsertTALEN Backbone
UsePhaeodactylum tricornutumTagsExpressionMutationPromoterAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pM9_fcpA_NG
Plasmid#90420PurposeTALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NGDepositorInsertTALEN Backbone
UsePhaeodactylum tricornutumTagsExpressionMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pM9_fcpA_NN
Plasmid#90419PurposeTALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NNDepositorInsertTALEN Backbone
UsePhaeodactylum tricornutumTagsExpressionMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pM9_fcpA_NI
Plasmid#90418PurposeTALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NIDepositorInsertTALEN Backbone
UsePhaeodactylum tricornutumTagsExpressionMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pM9_NR_NI
Plasmid#90422PurposeTALEN Backbone for Phaeodactylum tricornutum_ NR promotor_NIDepositorInsertTALEN Backbone
UsePhaeodactylum tricornutumTagsExpressionMutationPromoterAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPtGE34
Plasmid#107932PurposeExpresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugationDepositorInsertsOriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3
UseCRISPR and Synthetic Biology; Episomal vector for…TagsExpressionMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPtGE35
Plasmid#107999PurposeExpresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugationDepositorInsertsOriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3
I-TevI nuclease and partial linker domain
UseCRISPR and Synthetic Biology; Episomal vector for…TagsCas9ExpressionMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
HI.HslU.RSETB.fl.wt
Plasmid#12556DepositorInsertHaemophilus influenzae heat shock locus U
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
HI0058/pET-21a(+)c-tag
Plasmid#13097DepositorInsert3-deoxy-manno-octulosonate cytidylyltransferase (kdsB Haemophilus influenzae)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGS-21a-PcAad1
Plasmid#163039Purposeexpression of aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporiumDepositorInsertaryl-alcohol dehydrogenase
UseTagsGST and His6ExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
SGL40C.EFS.dTomato
Plasmid#89395PurposeAdvanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with dTomato expression (EFS Promoter)DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
L40C-CRISPR.EFS.dTomato
Plasmid#89392PurposeLentiviral CRISPR-Cas9 delivery for sgRNA (hU6), dTomato coexpression, EFS Promoter drivenDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
SGL40C-H1.EFS.RFP657
Plasmid#69148PurposeAdvanced lentiviral Vector for sgRNA (H1 Promoter) delivery with RFP657 expression (EFS Promoter)DepositorInsertssgRNA
EFS
tagRFP657
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only