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Plasmid#118856Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertEBFP2-LSSmOrange
UseTagsHis-tagExpressionBacterialMutationEBFP1.2-I128V/V150I/ D155V/V224R and for LSSmOra…PromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Fibrillarin-7
Plasmid#55241PurposeLocalization: Nucleoli, Excitation: 383, Emission: 448DepositorInsertFibrillarin (FBL Human)
UseTagsEBFP2ExpressionMammalianMutationR36Q, S66FPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EBFP2-Nucleus-7
Plasmid#55249PurposeLocalization: Nuclear Localization, Excitation: 383, Emission: 448DepositorInsertSV40 T-Antigen Nuclear Localization Signal (NLS)
UseTagsEBFP2-NLSExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
PB_TRE_AP4-PDGFR_EF1a_eBFP2
Plasmid#186306PurposePlasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.DepositorInsertsAP4-PDGFR
eBFP2
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-CENPB-N-22
Plasmid#55237PurposeLocalization: Nucleus/Centromeres, Excitation: 383, Emission: 448DepositorInsertCENPB (CENPB Human)
UseTagsEBFP2ExpressionMammalianMutationaa 1-169 of CENPB (DNA Binding Domain)PromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EBFP2-Vinculin-23
Plasmid#55252PurposeLocalization: Focal Adhesions, Excitation: 383, Emission: 448DepositorInsertVinculin (VCL Human)
UseTagsEBFP2ExpressionMammalianMutationV234L in VinculinPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EBFP2-Cx43
Plasmid#69020PurposeExpresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorInsertCx43 (Gja1 Rat)
UseTagsEBFP2ExpressionMutationPromoterCMVAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-VASP-5
Plasmid#55251PurposeLocalization: Actin/Focal Adhesions, Excitation: 383, Emission: 448DepositorInsertVASP (Vasp Mouse)
UseTagsEBFP2ExpressionMammalianMutationA209T in VASPPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRJPaph-eBFP2
Plasmid#67030PurposePlasmid for Paph-eBFP2 integration downstream of B. diazoefficiens (B. japonicum) scoIDepositorInsertB. diazoefficiens DNA from scoI downstream region (comprising blr1132')
UseConjugative bacterial vectorTagsExpressionMutationPromoterAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Cx30
Plasmid#69022PurposeExpresses human Cx30 (Gjb6 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorInsertCx30 (GJB6 Human)
UseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg88-PDGFR_EF1a_eBFP2
Plasmid#186312PurposePlasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.DepositorInsertssg88-PDGFR
eBFP2
UseTagsHis, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-EBFP2
Plasmid#184939PurposeER Visualization (OSER assay) Expresses EBFP2 in fusion with N-terminal part of p450DepositorInsertCytERM-EBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-CathespinB-6
Plasmid#55236PurposeLocalization: Lysosomes, Excitation: 383, Emission: 448DepositorInsertCathespinB
UseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EBFP2-UtrCH
Plasmid#124656PurposeF-actin marker. EBFP2 is fused to the calponin homology domain of utrophin (UtrCH).DepositorInsertUtrophin (UTRN Human)
UseTagsEBFP2ExpressionMammalianMutationsee depositor comments belowPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-CytERM-N-17
Plasmid#55238PurposeLocalization: Endoplasmic Reticulum, Excitation: 383, Emission: 448DepositorInsertCytERM
UseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EBFP2-LC-Myosin-N-7
Plasmid#55245PurposeLocalization: Light Chain Myosin, Excitation: 383, Emission: 448DepositorInsertLC-Myosin
UseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EBFP2-MAP4-C-10
Plasmid#55247PurposeLocalization: Microtubules, Excitation: 383, Emission: 448DepositorInsertMAP4
UseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 3, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EBFP2
Plasmid#167897PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD_EBFP2-ECFP
Plasmid#118854Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertEBFP2-ECFP
UseTagsHis-tagExpressionBacterialMutationEBFP1.2-I128V/V150I/ D155V/V224R and TGAGGGGLG be…PromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD_EBFP2-mTFP1
Plasmid#118855Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertEBFP2-mTFP1
UseTagsHis-tagExpressionBacterialMutationEBFP1.2-I128V/V150I/ D155V/V224R and TGAGGGGLG be…PromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only