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Plasmid#221526PurposeFlag-tagged biotinylation enzyme for co-expression of Psmd3DepositorInsertPSMD3 (PSMD3 Human)
UseTagsBirAExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
PSMC3_BirA_Nterm
Plasmid#221524PurposeFlag-tagged biotinylation enzyme for co-expression of Psmc3DepositorInsertPSMC3 (PSMC3 Human)
UseTagsBirAExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
SYNJ1_BirA_Nterm
Plasmid#221523PurposeFlag-tagged biotinylation enzyme for co-expression of Synj1DepositorInsertSYNJ1 (SYNJ1 Human)
UseTagsBirAExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
SYNJ1_BirA_Cterm
Plasmid#221522PurposeFlag-tagged biotinylation enzyme for co-expression of Synj1DepositorInsertSYNJ1 (SYNJ1 Human)
UseTagsBirAExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
PSMA4_BirA_Cterm
Plasmid#221513PurposeFlag-tagged biotinylation enzyme for co-expression of Psma4DepositorInsertPSMA4 (PSMA4 Human)
UseTagsBirAExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAPK9_BirA_Cterm
Plasmid#221520PurposeFlag-tagged biotinylation enzyme for co-expression of Mapk9DepositorInsertMAPK9 (MAPK9 Human)
UseTagsBirAExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2_BirA_NRF2
Plasmid#136524PurposeUsed as a donor vector to clone into pSLIKDepositorInsertNRF2 (NFE2L2 Human)
UseEntry vector for gateway cloningTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pVTU_260_BirA
Plasmid#63682PurposeEncodes E. coli biotin ligase gene (BirA) for expression in yeast cellsDepositorInsertBirA
UseTags6X Histidine and TEV cleavage siteExpressionYeastMutationPromoterADH1/yeastAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
K560_AviC_coBirA
Plasmid#129765PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assaysDepositorInsertKHC (Khc Fly)
UseTagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559PromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_BirA_CDKN1A
Plasmid#136538PurposeLentiviral expression vector for an inducible BirA-CDKN1ADepositorInsertBirA-CDKN1A (CDKN1A )
UseLentiviral; Destinatioin vector for gateway cloni…TagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_BirA
Plasmid#136537PurposeLentiviral expression vector for an inducible BirADepositorInsertBirA
UseLentiviral; Destinatioin vector for gateway cloni…TagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
K560_AviN_coBirA
Plasmid#129766PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assaysDepositorInsertKHC (Khc Fly)
UseTagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559PromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLIK_hygro_BirA_NRF2
Plasmid#136539PurposeLentiviral expression vector for an inducible BirA-NRF2DepositorInsertBirA-NRF2 (NFE2L2 Human)
UseLentiviral; Destination vector for gateway cloningTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLew100_myc_BirA*
Plasmid#41716DepositorTypeEmpty backboneUseT. brucei expressionTagsExpressionMutationPromoterPARPAvailable sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2_BirA_CDKN1A
Plasmid#136523PurposeUsed as a donor vector to clone into pSLIKDepositorInsertCDKN1A (CDKN1A Human)
UseEntry vector for gateway cloningTagsExpressionMutationPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2_BirA
Plasmid#136521PurposeUsed as a donor vector to clone into pSLIKDepositorInsertBirA
UseEntry vector for gateway cloningTagsExpressionMutationR118G (highly promiscuous form)PromoterAvailable sinceJune 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2_BirA_NRF2
Plasmid#136524PurposeUsed as a donor vector to clone into pSLIKDepositorInsertNRF2 (NFE2L2 Human)
UseEntry vector for gateway cloningTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDisplay-BirA-ER
Plasmid#20856DepositorInsertBirA-ER
UseTagsExpressionMammalianMutationC-terminal ER targeting sequence (KDEL)PromoterAvailable sinceMay 7, 2009AvailabilityAcademic Institutions and Nonprofits only -
secretedBirA-8his
Plasmid#32408DepositorInsertBirA
UseTags8 His tagExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEntr_Myc-BirA-G3-ER
Plasmid#173814PurposePromiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage siteDepositorInsertMyc-BirA-G3-ER
UseGateway shuttle vectorTagsMycExpressionMutationPromoterAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only