1. p426 72Q GPD

   Plasmid

   #1183

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 72Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. pENTR-actin-R62D

   Plasmid

   #118379

   Purpose

   Gateway compatible donor vector with actin-R62D.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Tags

   Expression

   Mutation

   R62D

   Promoter

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Flag-Src

   Plasmid

   #118308

   Purpose

   Expression of mouse Src

   Depositor

   Philip Stork

   Article

   Schmitt et al Mol Cell. 2002 Jan;9(1):85-94.

   Insert

   Src (Src Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Flag-RapGAP

   Plasmid

   #118324

   Purpose

   Expression of RapGAP

   Depositor

   Philip Stork

   Article

   Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

   Insert

   RapGAP
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFLAG-CrmA

   Plasmid

   #11831

   Depositor

   Guy Salvesen

   Article

   Muzio et al J Biol Chem. 1997 Jan 31. 272(5):2952-6.

   Insert

   CrmA (CrmA Cowpox virus)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

5. hHSF1 K298R

   Plasmid

   #118362

   Purpose

   This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K298R

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HA-GFP1-10-actin-pcDNA4-TO

   Plasmid

   #118370

   Purpose

   Bimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Tags

   HA-GFP1-10

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO

   Available since

   May 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GFP-Epac1 CAAX

   Plasmid

   #118312

   Purpose

   Expression of human Epac1

   Depositor

   Philip Stork

   Article

   Wang et al Mol Cell Biol. 2006 Mar;26(6):2130-45. d

   Insert

   Epac1 CAAX (RAPGEF3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C-terminal 20aa of KiRas

   Promoter

   CMV

   Available since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSUPER-hHSF2 shRNA

   Plasmid

   #118346

   Purpose

   This plasmid expresses shRNA against hHSF2.

   Depositor

   Lea Sistonen

   Article

   Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

   Insert

   hHSF2 shRNA (HSF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Flag-Rap1b V12

   Plasmid

   #118323

   Purpose

   Expression of bovine Rap1b

   Depositor

   Philip Stork

   Article

   Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

   Insert

   Rap1b V12 (RAP1B Bovine)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   CMV

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Flag-Rap1b N17

    Plasmid

    #118322

    Purpose

    Expression of bovine Rap1b

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Insert

    Rap1b N17 (RAP1B Bovine)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S17N

    Promoter

    CMV

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Flag-Craf S338A

    Plasmid

    #118335

    Purpose

    Expression of human Craf

    Depositor

    Philip Stork

    Article

    Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

    Insert

    Craf S338A (RAF1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S338A

    Promoter

    CMV

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mCherry-Rap2b

    Plasmid

    #118320

    Purpose

    Expression of human Rap2b

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Insert

    Rap2b (RAP2B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p221z-AtMIR390a

    Plasmid

    #118388

    Purpose

    Entry clone containing AtMIR390a backbone (split by ccdB gene). Used to make artificial microRNA construct. For use in plants and compatible with the MultiSite Gateway system.

    Depositor

    Ari Pekka Mähönen

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Insert

    AtMIR390a backbone
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-Epac2

    Plasmid

    #118314

    Purpose

    Expression of mouse Epac2

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2008 Dec;28(23):7109-25.

    Insert

    Epac2 (Rapgef4 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hHSF1 S303A

    Plasmid

    #118363

    Purpose

    This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    S303A

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET23b-Casp9 C287A-His

    Plasmid

    #11830

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

    Insert

    Caspase 9 catalytic mutant (CASP9 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    C287A

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

17. Flag-Rap1b

    Plasmid

    #118325

    Purpose

    Expression of bovine Rap1b

    Depositor

    Philip Stork

    Article

    Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

    Insert

    Rap1b (RAP1B Bovine)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-Braf R188L

    Plasmid

    #118330

    Purpose

    Expression of human Braf

    Depositor

    Philip Stork

    Article

    Li et al J Biol Chem. 2013 Sep 20;288(38):27646-5

    Insert

    Braf R188L (BRAF Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R188L

    Promoter

    CMV

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-Rap1b E63

    Plasmid

    #118328

    Purpose

    Expression of bovine Rap1b

    Depositor

    Philip Stork

    Article

    Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

    Insert

    Rap1b E63 (RAP1B Bovine)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Q63E

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge