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Plasmid#194182PurposeExpresses MPP8-delta442-860_10xHisDepositorInsertMPHOSPH8 (MPHOSPH8 Human)
UseTags10xHisExpressionMammalianMutationMPP8-delta442-860PromoterCMVAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-MPP8-delta600-728-10xHis
Plasmid#194183PurposeExpresses MPP8-delta600-728_10xHisDepositorInsertMPHOSPH8 (MPHOSPH8 Human)
UseTags10xHisExpressionMammalianMutationMPP8-delta600-728 (4xANK deletion)PromoterCMVAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-UbC-3xNLS-mScarlet-I
Plasmid#191099PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-mScarlet-I
UseAAV and Synthetic BiologyTagsThree repeats of the nuclear localizzation seque…ExpressionMammalianMutationoptimized to human codon usagePromoterUbCAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-gRNA(SFTPCI73Tcorr)-SpCas9(BB)-2A-GFP
Plasmid#187647PurposeEncodes gRNA to correct the SFTPC I73T mutationDepositorInsertI73T gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shAtxn1
Plasmid#180387PurposeProducing AAV that encodes mouse Atxn1 shRNA with miR-E backboneDepositorInsertshAtxn1 (Atxn1 Mouse)
UseAAV and RNAiTagsExpressionMutationPromoterCBAAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-flag-RAP1B-N17-puro
Plasmid#156169PurposeLentiviral vector for expression of flag-RAP1B-N17, with puromycin selectionDepositorInsertRAP1B (RAP1B Bovine)
UseLentiviralTags2xFLAGExpressionMammalianMutationchanged Serine 17 to AsparaginePromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP2A-WT-puro
Plasmid#156170PurposeLentiviral vector for expression of RAP2A-WT, with puromycin selectionDepositorInsertRAP2A (RAP2A Human)
UseLentiviralTagsExpressionMammalianMutationwild type formPromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP2A-V12-puro
Plasmid#156171PurposeLentiviral vector for expression of RAP2A-V12, with puromycin selectionDepositorInsertRAP2A (RAP2A Human)
UseLentiviralTagsExpressionMammalianMutationchanged Glycine 12 to ValinePromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP2A-N17-puro
Plasmid#156172PurposeLentiviral vector for expression of RAP2A-N17, with puromycin selectionDepositorInsertRAP2A (RAP2A Human)
UseLentiviralTagsnoneExpressionMammalianMutationchanged Serine 17 to AsparaginePromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.EFS.eGFP.mArid3a-3'UTR-Mut
Plasmid#169298PurposeConstitutive overexpression a mutated 3'UTR of the murine Arid3a gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.DepositorInsertArid3a-3'UTR-Mut (Arid3a Mouse)
UseLentiviralTagsExpressionMutationMutations in miR-125b-2 and let-7c binding sitesPromoterEFSAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.EFS.eGFP.ARID3A-3'UTR-Mut
Plasmid#169296PurposeConstitutive overexpression a mutated 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.DepositorInsertARID3A-3'UTR-Mut (ARID3A Human)
UseLentiviralTagsExpressionMutationMutations in miR-125b-2 and let-7c binding sitesPromoterEFSAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
t1-405(del30)
Plasmid#166128PurposeExpression of human talin-1 head (residues 1-405) in E. coli. Contains 30 amino acid deletion in F1-loop. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertT1head1-405(del30) (TLN1 Human)
UseTagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 1-405, with 30 amino acid deletion in th…PromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
t1-405(T144E,T150E)
Plasmid#166130PurposeFor expression of human talin-1 head (residues 1-405) in E. coli. Includes mutations T144E and T150E. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertHuT1head1-405(T144E,T150E) (TLN1 Human)
UseTagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 1-405, mutations T144E and T150EPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
RG6-BAP1_E7-D192D
Plasmid#154024PurposeBichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 7 c.576C>T, p.D192D (Synonymous Mutation)DepositorInsertBAP1 Exon 7 Fused to DsRed1 and eGFP (BAP1 Human)
UseSplicing reporterTagsDsRed1, FLAG, SV40 NLS, and eGFPExpressionMammalianMutationBAP1 c.576C>T, p.D192DPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
RG6-BAP1_E11-G312G
Plasmid#154026PurposeBichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 11 c.936T>G, p.G312G (Synonymous Mutation)DepositorInsertBAP1 Exon 11 Fused to DsRed1 and eGFP (BAP1 Human)
UseSplicing reporterTagsDsRed1, FLAG, SV40 NLS, and eGFPExpressionMammalianMutationBAP1 c.936T>G, p.G312GPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMBP-parallel1-GK5_v2 (D30N+D280N+D443N)
Plasmid#134291PurposeExpresses GK5 (isoform 2)-D30N/D280N/D443N mutant in bacteriaDepositorInsertGk5 (Gk5 Mouse)
UseTagsMBPExpressionBacterialMutationchanged Aspartic Acids 30, 280 and 443 to Asparag…PromoterT7Available sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-C-Flag-GK5-toku (1-174)
Plasmid#134261PurposeLentivector encoding Flag-tagged toku form of GK5 (amino acids 1-174)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk5 amino acids 1-174PromoterCMVAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBP-parallel1-GK5_v2 (D30N+D280N)
Plasmid#134292PurposeExpresses GK5 (isoform 2)-D30N/D280N mutant in bacteriaDepositorInsertGk5 (Gk5 Mouse)
UseTagsMBPExpressionBacterialMutationchanged Aspartic Acids 30 and 280 to AsparaginePromoterT7Available sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-C-Flag-GK5 (416-534)
Plasmid#134267PurposeLentivector encoding Flag-tagged GK5 truncation (amino acids 416-534)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk5 amino acids 396-534PromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-C-Flag-GK5 (396-534)
Plasmid#134266PurposeLentivector encoding Flag-tagged GK5 truncation (amino acids 396-534)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk5 amino acids 396-534PromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only