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Plasmid#79902PurposeGene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor LocusDepositorInsert3xFLAG_Twin_Strep_EZH2
UseCRISPR and TALENTags3xFLAG_Twin_StrepExpressionMammalianMutationPromoterPGK1Available sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBT276
Plasmid#122607Purposeexpress CDA1-BE4max in mammalian cellsDepositorInsertCDA1-BE4max
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-ZnF/HIT1
Plasmid#15332DepositorInsertzinc finger, HIT domain containing 1 (ZNHIT1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET21c-IF3mt
Plasmid#31078DepositorInsertMature form human mitochondrial translational initiation factor 3 (MTIF3 Human)
UseTagsHisExpressionBacterialMutationIncludes coding sequence for the mature form. Ami…PromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa-VPR mini.-syn pA
Plasmid#99689PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signalDepositorArticleInsertdCas9
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
px459 sgFIP200
Plasmid#175024PurposeCRISPR-Cas9 plasmid targeting exon 3 of human FIP200.DepositorInsertRB1CC1 (RB1CC1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-attBr-Cre-rc-attP
Plasmid#51264PurposeComponent of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientationDepositorInsertattBr-Cre-rc-attP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-LBR-mRFP1-polyA
Plasmid#59749PurposeExpress LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.DepositorInsertLBR-mRFP1
UseTagsmRFP1ExpressionMammalianMutationaa 1-249 of LBRPromoterCMV and T7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBrain-GFP-shGL2
Plasmid#60004PurposeCo-expresses control GL2 shRNA and GFP.DepositorInsertEGFP
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Blast NT shRNA
Plasmid#123306PurposeExpression of a non-targeting shRNADepositorInsertNon-targeting shRNA
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 P186C
Plasmid#66173PurposeExpresses the P186C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationP186CPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
[Bla][IRES][GFP]
Plasmid#97193PurposeVector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.DepositorInsertsbeta-Lactamase
GFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LplA(W37I) Alternative coumarin PRIME ligase
Plasmid#25846DepositorInsertLplA-W37I
UseTagsFlag and HisExpressionMammalianMutationW37IPromoterAvailable sinceJuly 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pC002 - LshC2C2 locus into pACYC184
Plasmid#79151PurposeEndogenous LshC2c2 locus cloned into pACYC184DepositorInsertLshC2c2 locus
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-PT2A-GFP-iRFP670-Tdtomato
Plasmid#111813PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsGFP
iRFP670
Tdtomato
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPpT4- TEF2prom-TIR1F74G-FLAG
Plasmid#189727PurposeEnables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)DepositorInsertOsTIR1
UseTagsFLAGExpressionYeastMutationPromoterTEF2Available sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSW178 - pXPP
Plasmid#115987Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpXPP
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+MT-hToca-1-W518K
Plasmid#33031DepositorInsertToca-1 (FNBP1L Human)
UseTags6xMycExpressionMammalianMutationW518KPromotersimian CMV IE94Available sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-tdTomato
Plasmid#125573PurposeCre-dependent expression of tdTomato in mammalian cells.DepositorInserttdTomato
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
piggybac-Syn-mScn8a-3xHA
Plasmid#182555PurposeExpresses Nav1.6 tagged with 3xHADepositorInsertScn8a (Scn8a Mouse)
UseTags3xHAExpressionMammalianMutationPromoterSynAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only