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Plasmid#80331PurposeExpresses Danio rerio VSP (without phosphatase activity) with separated EGFP in mammalian cellDepositorInsertDaino rerio voltage-sensing phosphatase (tpte Zebrafish)
UseTagsExpressionMammalianMutationC302SPromoterCMVAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab4a-7
Plasmid#56440PurposeLocalization: Ras GTPase, Excitation: 488, Emission: 507DepositorInsertRab4a (RAB4A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pAAV-CaMKII-GAL4-iLight-VP16
Plasmid#170274PurposeNLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.DepositorInsertNLS-Gal4(1-65)-iLight(human codon optimized)-VP16
UseAAVTagsExpressionMutationSee CommentsPromoterCaMKIIAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-BFP
Plasmid#107722PurposeFor expression of sgRNA from the U6 promoter with BFP expression simultaneouslyDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-HcKCR1-EYFP
Plasmid#182021PurposeExpress light-gated potassium channel HcKCR1-EYFP in mammalian cellsDepositorInsertHcKCR1
UseAAVTagsEYFPExpressionMammalianMutationPromoterAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AC EJ7-GFP
Plasmid#113617PurposeReporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFPDepositorInsertEJ7-GFP variant of EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-3xFLAG-GFP-puroR
Plasmid#167463Purpose3rd-gen lentiviral expression of FLAG-GFP in mammalian cellsDepositorInsertGFP
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC3.1-caggs-Crimson-CAAX
Plasmid#190041PurposeRed Fluorescent Protein for Long-Term Labeling of Fine Structures in NeuronsDepositorInsertCrimson
UseTagsCAAXExpressionMammalianMutationPromotercaggsAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCMV-H2B-FT-Medium
Plasmid#157670PurposeRetroviral vector for expressing chimeric human histone H2B fused with the "Medium" variant of the monomeric color-changing fluorescent timer (FT) in mammalian cells.DepositorInsertHistone H2B
UseRetroviralTagsMedium-FTExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP2-96-merTetO-EFS-BLaR
Plasmid#118713PurposeContains an optimized 96-mer TetO repeat for imaging of desired lociDepositorInsertsBlasticidin resistance gene
EFS-NS promoter
UseTagsExpressionMammalianMutationPromoterEFS-NSAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJRH051
Plasmid#171625Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-BsmBI-Destination
mNeon
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1-a and U6Available sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-mPGK-eGFP (beta-globin intron)
Plasmid#162513PurposeAAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (mPGK) promoter that is upstream of a beta-globin intronDepositorInserteGFP
UseAAVTagsExpressionMammalianMutationPromotermPGKAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast
Plasmid#138149PurposeExpresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleusDepositorInsertCasRx
UseLentiviralTagsNLS-HAExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-RO1.7-RPE65
Plasmid#206346PurposeAAV plasmid expressing RPE65 in photoreceptorsDepositorInsertRpe65 (Rpe65 Mouse)
UseAAVTagsExpressionMutation450-Met (C57BL6 natural variation)Promoter1.7kb human red opsinAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE
Plasmid#138151PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR_BsmBI
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Large BiT - SNAP-tag - Beta-actin
Plasmid#171019PurposeLentiviral expression of a Large BiT-SNAP-tag-Beta-actin fusion in mammalian cellsDepositorInsertLargeBit-SNAPtag- Beta-actin
UseLentiviral and LuciferaseTagsBeta actin and LargeBiTExpressionMammalianMutationPromoterEF1Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE312B
Plasmid#128969PurposeBroad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid inductionDepositorInsertsPapRecT
mutL E32K
UseTagsExpressionBacterialMutationE32KPromoterxylS-PmAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-EGFP
Plasmid#107721PurposeFor expression of sgRNA from the U6 promoter with EGFP expression simultaneouslyDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMPE-ePPEplus
Plasmid#205242PurposeFor multiplex plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertCsy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only