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Plasmid#205579PurposeKAN(1-192)-NpuDnaE(N)_GFPuvDepositorInsertNpuDnaE(N)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0009
Plasmid#205578PurposeNpuDnaE(C)_KAN(132-265)_RUBYDepositorInsertNpuDnaE(C)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0008
Plasmid#205577PurposeKAN(1-131)-NpuDnaE(N)_GFPuvDepositorInsertNpuDnaE(N)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0007
Plasmid#205576PurposeNpuDnaE(C)-Glucos(232-500)DepositorInsertNpuDnaE(C)-Glucosyltransferase
UseTagsExpressionPlantMutationPromoter35SAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0018
Plasmid#205587Purposep3flag_HygR(1-52)-NpuDnaE(N)DepositorInsertHygR-NpuDnaE(N)_homo-codon-OP
UseTags3xFLAGExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0019
Plasmid#205992PurposepNpuDnaE(C)_HygR(53-341)_3HADepositorInsertNpuDnaE(C)-HygR
UseTags3xHAExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6-2xmNeon
Plasmid#178462PurposeA tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag in pET28a.DepositorInsertmNeonGreen dimer
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTatABmNeonC
Plasmid#178464PurposepTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatB.DepositorInsertTatA, TatBmNeon, TatC
UseTagsmNeonGreen fused to TatBExpressionBacterialMutationPromoteraraCAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTatABCmNeon
Plasmid#178465PurposepTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatC.DepositorInsertTatA, TatB, TatCmNeon
UseTagsmNeonGreen fused to TatCExpressionBacterialMutationPromoteraraCAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTatAkBxCs
Plasmid#178544PurposepTatABC with KpnI, XbaI, and KpnI restriction sites after TatA, TatB, and TatC, respectively.DepositorInsertTatA, TatB, TatC
UseTagsExpressionBacterialMutationPromoteraraCAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD_TatABmCherryC
Plasmid#178545PurposepTatABC with an mCherry fluorescent protein tag at the end of TatB.DepositorInsertTatA, TatBmCherry, TatC
UseTagsmCherry fused to TatBExpressionBacterialMutationPromoteraraCAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
RS314-U6(PolII-Lsm)
Plasmid#200772PurposePol II expression of U6 LsmDepositorInsertGal-SNR6-II
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-leuYFP[CTT]-nLuc
Plasmid#199757PurposeElongation reporter construct to quantify elongation of synYFP[CTT]DepositorInsertleuYFP[CTT]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-leuYFP[CTC]-nLuc
Plasmid#199758PurposeElongation reporter construct to quantify elongation of synYFP[CTC]DepositorInsertleuYFP[CTC]
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK BirA* zeo
Plasmid#192332PurposeLentiviral expression vector for an inducible 3xFLAG-BirADepositorInsertBirA*
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT BirA* w ccdB
Plasmid#192310PurposeGateway entry vector for cloningDepositorInsertBirA*
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-Linker
Plasmid#192305PurposeGateway entry vector for cloningDepositorInsertLinker
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFV*/Puro2a-PAG1-GFP
Plasmid#198223PurposeLentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFPDepositorInsertPAG1 (PAG1 Human)
UseLentiviralTagsFollowed by EGFP, after a flexible linker and Pre…ExpressionMammalianMutationNonePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAD424-AP-4 σ4
Plasmid#198328PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-4 σ4 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-4 σ4
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
SL-TREAT
Plasmid#196918PurposeThe TREAT reporter containing cap-proximal GC-rich stemloopDepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only