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Plasmid#185240PurposeFor the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k3_1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).DepositorInsertDrHD_dom3_k3_1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_CI016_HUMAN (pBS0747)
Plasmid#185238PurposeFor the mammalian expression of the human protein CI016_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertCI016_HUMAN
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_AdAPOA4-44 (pBS0745)
Plasmid#185236PurposeFor the mammalian expression of the Chinese giant salamander protein AdAPOA4-44. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).DepositorInsertAdAPOA4-44
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA0057
Plasmid#131784PurposeGuide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.DepositorInsert20mer GFP cassette guide (gGFP) and 5' sgRNA
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-cytXlATL2
Plasmid#124063PurposeFragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.DepositorInsertcytXIATL2
UseTagsExpressionBacterialMutationPromotertacAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1474
Plasmid#82420PurposePlasmid for expression of HA-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cellsDepositorInsertnvGW182 NED
UseTagsHAExpressionMammalianMutationdeleted amino acids 1159-1698 from nvGW182PromoterCMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1475
Plasmid#82421PurposePlasmid for expression of HA-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cellsDepositorInsertnvGW182 CED
UseTagsHAExpressionMammalianMutationdeleted amino acids 1-1158 from nvGW182PromoterCMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1476
Plasmid#82422PurposePlasmid for expression of NHA-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cellsDepositorInsertnvGW182 NED
UseTagsNHAExpressionMammalianMutationdeleted amino acids 1159-1698 from nvGW182PromoterCMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1477
Plasmid#82423PurposePlasmid for expression of NHA-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cellsDepositorInsertnvGW182 CED
UseTagsNHAExpressionMammalianMutationdeleted amino acids 1-1158 from nvGW182PromoterCMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1479
Plasmid#82425PurposePlasmid for expression of GST-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cellsDepositorInsertnvGW182 NED
UseTagsGSTExpressionMammalianMutationdeleted amino acids 1159-1698 from nvGW182PromoterEF1AAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1480
Plasmid#82426PurposePlasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cellsDepositorInsertnvGW182 CED
UseTagsGSTExpressionMammalianMutationamino acids 1-1158 of nvGW182 were deletedPromoterEF1AAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
tdminiSOG-Tubulin-6
Plasmid#57808PurposeLocalization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertTubulin (TUBA1B Human)
UseTagstdminiSOGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
PA-mCherry-miniSOG-Vinculin-23
Plasmid#57801PurposeLocalization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertVinculin (VCL Human)
UseTagsPA-mCherry-miniSOGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
miniSOG-Vinculin-23
Plasmid#57780PurposeLocalization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertVinculin (VCL Human)
UseTagsminiSOGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCherry-miniSOG-Zyxin-6
Plasmid#55101PurposeLocalization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertZyxin (ZYX Human)
UseTagsmCherry-miniSOGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
1287 p3E-mut let-7 etv2 3’ UTR
Plasmid#49011PurposeZebrafish Long length etv2 3' UTR with five let7 binding site mutations in gateway 3' Entry vectorDepositorInsertets variant gene 2 (etsrp Zebrafish)
UseGateway 3' entryTagsExpressionMutationMutated the Let7 miRNA binding sitesPromoterAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLdCN
Plasmid#84290PurposeExpress gRNA and Cas9 in Leishmania with Neomycin resistanceDepositorInsertgRNA and Cas9
UseCRISPR; LeishmaniaTagsExpressionMutationPromoterL. donovani ribosome RNA promoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
LBR pEGFP-N2 (646)
Plasmid#61996Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertLBR (LBR Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa (T286D)
Plasmid#127391PurposeFluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D)DepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
UseTagsmEGFPExpressionMammalianMutationchanged Threonine 286 to Aspartic acidPromoterpCAGAvailable sinceJan. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
XIAP (pBS0852)
Plasmid#185282PurposeFor the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay.DepositorInsertXIAP (XIAP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only