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Plasmid#164632PurposeExpresses RAB35 in mammalian cells with a 2xmyc tagDepositorInsertRAB35 (RAB35 Human)
UseTags2xmycExpressionMammalianMutationnonePromoterCMVAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF-Bos TRAM Flag
Plasmid#41551DepositorInsertTRAM (TICAM2 Human)
UseTags6xHis and FlagExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-T2A-mCherry-PTS1
Plasmid#87827PurposeMammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMV promoter, tetracycline operatorAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NMHC-IIA-3xA
Plasmid#101041PurposeExpresses GFP-MYH9 construct (human myosin IIA) carrying S1943A mutation and S1916A, S1915A mutation, driven by CMV promoter. Thus inhibits CKII and PKC phosphorylation of the tail.DepositorInsertNon-muscle myosin IIA heavy chain (MYH9 Human)
UseTagsGFPExpressionMammalianMutationS1915A, S1916A, S1943APromoterCMVAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shGBP1.1.mKO2
Plasmid#85209PurposeTRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.DepositorInsertGBP1 (guanylate binding protein 1) (GBP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
p28-2iDP-puro
Plasmid#88893PurposeDual-intron DMD platform plasmid. Co-expresses DMD platform segment, firefly luciferase, puroR and mCherry. Plasmid carries phiC31 and Bxb1 attB sites.DepositorInsertsDual-intron DMD platform
luciferase
puromycin resistance enzyme
mCherry
UseTagsExpressionMammalianMutationTruncated version of the dystrophin protein (tran…PromoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]
Plasmid#74102Purposeplasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)DepositorInsertsXenopus laevis beta-tubulin gene promoter region and 5'UTR
Green Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYpet-Optop38i
Plasmid#89749PurposeExpression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13FDepositorInsertOptop38i3
UseFluorescent proteinTagsYpetExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pRB051_LEU2
Plasmid#196993PurposeBig-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.DepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and Synthetic Biology ;…TagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311 ETV4-S
Plasmid#74983PurposeConstitutive ETV4 ORF, blasticidin selectionDepositorInsertETV4 (ETV4 )
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 S303A
Plasmid#118363PurposeThis plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationS303APromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
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Flag-M4-AR-S81A
Plasmid#171241PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Ala (AR-S81A)DepositorInsert3xFlag-tagged human androgen receptor Ser81Ala mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Ser81Ala (AR-S81A)PromoterCMVAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-S81D
Plasmid#171242PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Asp (AR-S81D)DepositorInsert3xFlag-tagged human androgen receptor Ser81Asp mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Ser81Asp (AR-S81D)PromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shETV1-A
Plasmid#74973PurposeshETV1 shRNADepositorInsertETV1 (ETV1 Human)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMVA2
Plasmid#119951PurposeExpresses recombinant MVA pathway in Escherichia coliDepositorInsertsmvaE
mvaS
mvaK1
mvaK2
mvaD
idi
UseTagsExpressionBacterialMutationA110GPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 shETV1-B
Plasmid#74974PurposeshETV1 shRNADepositorInsertETV1 (ETV1 Human)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
sgAAVS1(A)
Plasmid#85570PurposeExpresses sgRNA targeting human AAVS1 locusDepositorInsertadeno-associated virus integration site 1 (AAVS1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUMO His6 SUMO SnRK2.2 (20-362)
Plasmid#177845PurposeBacterial Expression of SnRK2.2DepositorInsertSNF1-related protein kinase 2 (SNRK2.2 Mustard Weed)
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only