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Plasmid#111512PurposeElectrophysiology in HEK cellDepositorInsertcalmodulin [Xenopus laevis] (calm2 Frog)
UseTagsExpressionMammalianMutationD20A/D56APromoterAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
ME2/pBABE-puro
Plasmid#49164PurposeFor expression of Malic Enzyme 2 in mammalian cells from retroviral vectorDepositorInsertMalic Enzyme 2 (ME2 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEN-R2-mCsfGFP-L3
Plasmid#178602PurposeIt can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.DepositorInsertTandem fluorescent protein timers (tFTs)
UseEntry vector for gateway cloningTagsmCherry superfolder GFPExpressionMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQC.Flip-nluc(LgBiT1-8)CP[Casp3A]-Fluc
Plasmid#200123PurposeRetroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A] protease reporter and P2A firefly luciferase in mammalian cellsDepositorInsertFlip-nluc(LgBiT1-8)CP[Casp3A] P2A firefly luciferase
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynWSyt1C2AB(80 JuxtaK-421)msGFP
Plasmid#209016PurposeOverexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cellsDepositorInsertsyt1 (Syt1 Rat)
UseLentiviralTagsmsfGFPExpressionMammalianMutationaa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKEā¦PromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQC.Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc
Plasmid#200125PurposeRetroviral expression of Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cellsDepositorInsertFlip-nluc(LgBiT1-8)CP[CoVA] P2A firefly luciferase
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TdIF2
Plasmid#199699Purposevisualization of nucleolus in living mammalian cellsDepositorInsertTdIF2
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-bphD
Plasmid#128138PurposeThe cell-free adapted backbone, pBEAST, expressing bphD (the enzyme converting 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertbphD
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET16b-PCNA
Plasmid#134898PurposeBacterial expression and purification of human PCNADepositorInsertPCNA (PCNA Human)
UseTagsuntaggedExpressionBacterialMutationfully synthetic, codon optimized for bacterial exā¦PromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
Daxx/pEGFP
Plasmid#119021PurposeExpress GFP-Daxx in mamalian cellsDepositorInsertdeath-domain associated protein (Daxx) (DAXX Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW-tTRE-scFv-SunTag
Plasmid#193138PurposeExpresses scFv intracellular antibody component of the SunTag system under Dox regulationDepositorInsertscFv-SunTag
UseLentiviralTagsExpressionMammalianMutationPromotertight TRE promoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pNPK-NLS-Vika
Plasmid#79969Purposeexpresses human codon-optimized Vika-recombinase in mammalian cellsDepositorInserthuman codon-optimized Vika sequence, NLS tagged
UseTagsNLSExpressionMammalianMutationPromoterPGKAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_UGControl
Plasmid#133875PurposeExpresses UGControl in mammalian cellsDepositorInsertUtrCH (UTRN Human)
UseTagsflexible linker - mEGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_UG3
Plasmid#133874PurposeExpresses UG3 in mammalian cells.DepositorInsertUtrCH (UTRN Human)
UseTagshelical linker - mEGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-SnFR-gamma2-minWPRE
Plasmid#165497PurposeAAV for glutamate reporter fused to TARP gamma-2 for postsynaptic localisationDepositorInsertiGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Rat, Synthetic)
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_TCF4_WT
Plasmid#82166PurposeGateway Donor vector containing TCF4 , part of the Target Accelerator Plasmid Collection.DepositorInsertTCF4 (TCF4 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
STIMATE(1-240)
Plasmid#71280PurposeExpresses STIMATE or TMEM110 (1-240) in mammalian cellsDepositorInsertSTIMATE (1-240) (TMEM110 Human)
UseTagsmcherryExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-NONO
Plasmid#127655PurposeOver-express HA-tagged human NONODepositorInsertNONO (NONO Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF2747
Plasmid#143606PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNKX6-2 (NKX6-2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only