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Plasmid#194696PurposeHuC driven expression of the calcium recorder Caprola_01 fused to mEGFP for neuronal expression in zebrafishDepositorInsertCaprola_01-mEGFP
UseZebrafish expressionTagsmEGFPExpressionMutationPromoterelavl3(HuC)Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
hsp70l: hAPOB48-mCherry.
Plasmid#140639PurposeHeat shock promoter driven expression of human APOB48-mCherry. The construct has tol2 elements, and is designed to use in zebrafish.DepositorUseZebrafish expressionTagsmCherryExpressionMutationAPOB48: amino acid 1-2180Promoterhsp70lAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-NLS-BirA-2A-mCherry_Ras
Plasmid#80067PurposeBeta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-cytoBirA-2A-mCherry_Ras
Plasmid#80066PurposeBeta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5E-1.7kbfabp6
Plasmid#159087Purposep5E with promoter sequence 1.7kb upstream of zebrafish fabp6 geneDepositorInsert1.7kb fabp6 promoter (fabp6 Zebrafish)
UsePromoter fragment 5' entry vector for tol2 c…TagsExpressionMutationPromoter1.7kb fabp6Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-myl7-cytoBirA-2A-mCherry_Ras
Plasmid#80060PurposeMyl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterMyl7 promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-EGFP
Plasmid#164940PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminusDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsEGFPExpressionMutationStop codon deletedPromoteractinb (actb2) promoterAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT-zic2a-cytoBirA-2A-mCherry_Ras
Plasmid#80064PurposeZic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterZic2a enhancer driving c-fos promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-MCS-NLS-BirA-2A-mCherry_Ras
Plasmid#80059PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-zic2a-NLS-BirA-2A-mCherry_Ras
Plasmid#80065PurposeZic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterZic2a enhancer driving c-fos promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-pax3a-kcnj13-IRES-EGFP
Plasmid#164950PurposeTol2 construct: pax3a-5.4k promoter driven zebrafish kcnj13 fused with EGFP and EGFPDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoterpax3a promoter -5.4kAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-IRES-EGFP
Plasmid#164941PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 and EGFPDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoteractinb (actb2) promoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-RanGAP-Cerulean-Avi
Plasmid#80068PurposeBeta-actin2 promoter driving C-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
UseZebrafish biotaggingTagsAviExpressionBacterialMutationPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-myl7-NLS-BirA-2A-mCherry_Ras
Plasmid#80061PurposeMyl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterMyl7 promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoEa
Plasmid#121900PurposePlasmid for making zebrafish apoEa in situ probeDepositorInsertApoEa (apoea Zebrafish)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-drN-hsAgo2
Plasmid#221724PurposeExpression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for zebrafish Ago2 N-domain.DepositorInsertdrN-hsAgo2 (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationSwap of N-domain for zebrafish Ago2 N-domainPromotersimian CMV IE94Available sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2_elavl3(HuC)_NES-Caprola_on-mEGFP
Plasmid#194697PurposeHuC driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression in zebrafishDepositorInsertCaprola_on-mEGFP
UseZebrafish expressionTagsmEGFPExpressionMutationPromoterelavl3(HuC)Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2_elavl3(HuC)_NES-Caprola_off-mEGFP
Plasmid#194698PurposeHuC driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression in zebrafishDepositorInsertCaprola_off-mEGFP
UseZebrafish expressionTagsmEGFPExpressionMutationPromoterelavl3(HuC)Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cox3-FusXTBE-Left TALE
Plasmid#196504PurposeExpresses left-side TALE–DddAtox N term half cox3-FusXTBE in zebrafishDepositorInsertIDH2 MTS-cox3 left TALE-G1397 DddA-C-UGI-SV40 polyA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cox1-FusXTBE-Left TALE
Plasmid#196502PurposeExpresses left-side TALE–DddAtox N term half cox1-FusXTBE in zebrafishDepositorInsertIDH2 MTS-cox1 left TALE-G1397 DddA-C-UGI-SV40 polyA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only