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Showing: 561 - 580 of 72587 results

  4. pSB1A3 EcoRI Methylase-AmilCP

    Plasmid
    #85166
    Purpose
    For methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.
    Depositor
    Robin Dowell
    Article
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI Methylase-AmilCP
    Use
    Tags
    AmilCP
    Expression
    Bacterial
    Mutation
    Promoter
    J23100 promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. pSC-cUnaG-LPETG

    Plasmid
    #207635
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Amber stop codon at SC's critical lysine
    Promoter
    T7
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207635-plasmid-map-sequence-id-409865

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  7. pGL4_CRE-CMVmin-luc2

    Plasmid
    #194384
    Purpose
    Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter
    Depositor
    Michael Wehr
    Article
    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48
    Insert
    6xCRE-CMVmin-luc2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV minimal
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    194384-plasmid-map-sequence-id-381615

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  8. EGFP-ST-P2A-pSC-CAAX

    Plasmid
    #207637
    Purpose
    A plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    EGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Amber stop codon at SC's critical lysine
    Promoter
    CMV
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207637-plasmid-map-sequence-id-409879

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  20. pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh

    Plasmid
    #207634
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Amber stop codon at SC's critical lysine
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207634-plasmid-map-sequence-id-413133

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Showing: 561 - 580 of 72587 results