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Plasmid#212935PurposeRapidResponse(TM) Renilla luciferase vector for investigating regions controlling transcriptionDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationWTPromoterAvailable sinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
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pGL4.83(hRlucP/Puro)
Plasmid#212934PurposeRenilla luciferase+PEST vector for investigating faster response to transcriptional changesDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationWTPromoterAvailable sinceJan. 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
LentiV_Cas9_puro
Plasmid#108100PurposeLentiviral expression plasmid of spCas9 with puromycin resistance geneDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS promoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEFBOS_CreIRESpuro
Plasmid#183812PurposeExpresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoterDepositorInsertCre recombinase
UseSynthetic BiologyTagsNLSExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EGFP-IRES-puro
Plasmid#128652Purposemammalian expression of EGFP with puromycin selectionDepositorInsertEGFP
UseLentiviralTagsN/AExpressionMammalianMutationWild typePromoterCMVAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRDA_052
Plasmid#136474PurposeLentiviral expression of AsCas12a gRNADepositorInsertPuromycin resistance
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterEF1aAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-MCS-GFP-SV-puro
Plasmid#73582PurposeThird generation lentiviral vector expressing GFP and puromycinDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsGFP tagExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-CAG-mCh
Plasmid#80492Purposedonor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expressionDepositorInsertmCherry
UseDonor vector (human)TagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-NORAD
Plasmid#196086PurposeInducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting lncRNA NORAD.DepositorInsertgRNA targeting lncRNA NORAD
UseCRISPRTagsExpressionMammalianMutationCloned using SapI sites - destroyed after inserti…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAIP hIL2 co
Plasmid#90513PurposeLentiviral vector for expression of human codon optimized IL2 with puromycin resistance.DepositorInsertIL2 (IL2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-HIV-1 Group P-vpr
Plasmid#115837PurposeEncodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance proteinDepositorInsertHIV1 Group P Vpr (HQ179987)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST ERKKTRClover
Plasmid#90227PurposeLentiviral vector to express ERK KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertERK Kinase Translocation Reporter
UseLentiviralTagsmCloverExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SlugCas9-NNG-P2A-puro
Plasmid#214352PurposeExpresses SlugCas9-NNG and puromycin resistance genesDepositorInsertSlugCas9-NNG and puromycin resistance genes
UseTagsExpressionMammalianMutationQ782R/S888R/L906R/N984S/E1012K/K1016IPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cas9-sgRNA-A
Plasmid#149369PurposeExpresses SpCas9 and sgRNA targeting the lenti-CDDR reporterDepositorInsertsCas9
sgRNA-A
Puromycin resistance
UseCRISPRTags3xFLAG, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterCBh, PGK, and U6Available sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-HIV-1 Group O-vpr
Plasmid#115836PurposeEncodes codon optimized 3xFLAG-HIV1 Group O Vpr (L20571) and puromycin resistance proteinDepositorInsertHIV1 Group O Vpr (L20571)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRosetta_v2
Plasmid#136477PurposeLentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycinDepositorInsertHygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SlugABE-NNG-P2A-puro
Plasmid#214354PurposeExpresses SlugABE-NNG and puromycin resistance genesDepositorInsertSlugABE-NNG and puromycin resistance genes
UseTagsExpressionMammalianMutationQ782R/S888R/L906R/N984S/E1012K/K1016IPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM60 FLAG-RAB35 wt
Plasmid#107104PurposeLentiviral vector that codes for FLAG-RAB35 wildtype (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.DepositorInsertRAB35 (RAB35 Human)
UseLentiviralTagsFLAG tagExpressionMammalianMutationwildtypePromoterCMVAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-MRE11-H129N
Plasmid#82034PurposePlasmid for constitutive or doxycycline-inducible expression of H129N nuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertMRE11 (MRE11 Human)
UseTagsHAExpressionMammalianMutationNuclease-dead mutant: H129N MRE11PromoterCMV-tetAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pICE-NLS-mCherry
Plasmid#60364PurposePlasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertNLS-mCherry
UseTagsNLS from SV40 T antigen and mCherryExpressionMutationPromoterCMV-tetAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only