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Plasmid#170088Purposeencodes E. coli type I-E single spacer arrayDepositorInsertE. coli type I-E single spacer array
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterJ23119Available sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR mCherry L1-R5
Plasmid#43963DepositorInsertmCherry
UseMultisite gateway entr cloneTagsExpressionMutationPromoterAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
mRFP1-9
Plasmid#63818PurposeA member of an RBS library for mRFP1 with different translation ratesDepositorInsertmRFP1
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterJ23100Available sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5T6T,P-J23106-geneIII
Plasmid#80863PurposePhagemid (encodes evolved cI4A5T6T,P and M13 gene III)DepositorInsertsM13 gene III
cI4A5T6T,P
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5C6G7G-J23106-geneIII
Plasmid#80909PurposePhagemid (encodes cI4A5C6G7G and M13 gene III)DepositorInsertsM13 gene III
cI4A5C6G7G
UseTagsExpressionBacterialMutation238G to 238D (please see depositor comment below)PromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
mRFP1-1
Plasmid#63816PurposeA member of an RBS library for mRFP1 with different translation ratesDepositorInsertmRFP1
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterJ23100Available sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR eGFPCRE L1-R5
Plasmid#43960DepositorInserteGFPCre
UseMultisite gateway entr cloneTagsExpressionMutationPromoterAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR IRES L5-L4
Plasmid#43964DepositorInsertIRES
UseMultisite gateway entr cloneTagsExpressionMutationPromoterAvailable sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A2T
Plasmid#62258Purposeexpression of A2T sgRNA from the arabinose-inducible promoterDepositorInsertA2T sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR pA L3-L2
Plasmid#43966DepositorInsertpA
UseMultisite gateway entr cloneTagsExpressionMutationPromoterAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A1T
Plasmid#62256Purposeexpression of A1T sgRNA from the arabinose-inducible promoterDepositorInsertA1T sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A4NT
Plasmid#62261Purposeexpression of A4NT sgRNA from the arabinose-inducible promoterDepositorInsertA4NT sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A3T
Plasmid#62260Purposeexpression of A3T sgRNA from the arabinose-inducible promoterDepositorInsertA3T sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A3NT
Plasmid#62259Purposeexpression of A3NT sgRNA from the arabinose-inducible promoterDepositorInsertA3NT sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAGD248
Plasmid#219498PurposeTemperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)DepositorInsertEco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL391
Plasmid#219501PurposeExpress Ade2 and Faa1 editrons from a single Gal7 promoter, separated by a Csy4 recognition sequenceDepositorInsertEco1 editing ncRNAs and Cas9 gRNAs to edit ADE2 and FAA1
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRC043
Plasmid#206799PurposeJ23107.SoxS-GID1DepositorInsertSoxS-GID1
UseTagsExpressionMutationPromoterJ23107Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRC025
Plasmid#206796PurposeJ23107.MCP-ABIDepositorInsertMCP-ABI
UseTagsExpressionMutationPromoterJ23107Available sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRC011
Plasmid#206794PurposeJ23107.MCP-SYNZIP6DepositorInsertMCP-SYNZIP
UseTagsExpressionMutationPromoterJ23107Available sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRC012
Plasmid#206795PurposeJ23107.SoxS-SYNZIP5DepositorInsertSoxS-SYNZIP
UseTagsExpressionMutationPromoterJ23107Available sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only