Addgene

We narrowed to 1,014 results for: gibson assembly

Showing: 561 - 580 of 1014 results

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TOPO Cre-2A-GFP

Plasmid

#68450

Purpose

TOPO construct expressing Cre-2A-GFP for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Cre-2A-GFP

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pTRE:CloverCP-EFS:ZeoR

Plasmid

#68478

Purpose

Lentivirus for establishment of doxycycline-inducible cell line expression CloverCP

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

CloverCP
Zeocin Resistance

Use

Lentiviral

Tags

"CP" degradation domain

Expression

Mammalian

Mutation

Promoter

EFS and pTRE

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-pGK:GFP

Plasmid

#68486

Purpose

Retrovirus expressing GFP driven by pGK promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR and pGK

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO pTRE

Plasmid

#68457

Purpose

TOPO construct expressing pTRE for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Tetracycline Response Element Promoter

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pTRE:shRNA A-pGK:rtTA-Cre

Plasmid

#68471

Purpose

Inducible lentivirus expressing an arbitrary shRNA and Cre

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

GFP-shRNA A-miR30
rtTA-2A-Cre

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

pGK and pTRE

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_h7SK

Plasmid

#102842

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_h7SKpromoter

Use

As pcr template

Tags

Expression

Mutation

Promoter

h7SK

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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STAGR_SAMScaffold_mU6

Plasmid

#102845

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert SAM_mU6

Use

Pcr template for stagr inserts

Tags

Expression

Mutation

Promoter

mU6

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-UBC:GFP

Plasmid

#68482

Purpose

Retrovirus expressing GFP driven by UBC promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR and UBC

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO EFS

Plasmid

#68451

Purpose

TOPO construct expressing EFS for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Elongation Factor 1α Promoter

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-SV40:GFP

Plasmid

#68483

Purpose

Retrovirus expressing GFP driven by SV40 promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR and SV40

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO CCSP

Plasmid

#68453

Purpose

TOPO construct expressing CCSP for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Clara Cell Secretory Protein Promoter

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO SV40

Plasmid

#68455

Purpose

TOPO construct expressing SV40 for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Simian Virus Early 40 Promoter

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-EFS:GFP

Plasmid

#68484

Purpose

Retrovirus expressing GFP driven by EFS promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS and LTR

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pNS3

Plasmid

#131770

Purpose

PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_FUSN_Fusion Red_Cry2Olig_TACT1

Use

Integration into δ-sites

Tags

Expression

Yeast

Mutation

E490G to make Cry2 into Cry2Olig

Promoter

PADH1

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pNS1

Plasmid

#131769

Purpose

PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

FUSN_Fusion Red_Cry2WT

Use

Integration into δ-sites

Tags

Expression

Yeast

Mutation

Promoter

PADH1

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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FFiBlast MSCV Puro

Plasmid

#68464

Purpose

Retroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Blasticidin Resistance

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Inverted

Promoter

LTR

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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STAGR_SAMScaffold_hH1

Plasmid

#102847

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert SAM_hH1

Use

Pcr template for stagr inserts

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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STAGR_SAMScaffold_h7SK

Plasmid

#102846

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert SAM_h7SK

Use

Pcr template for stagr inserts

Tags

Expression

Mutation

Promoter

H7SK

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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TOPO CMV

Plasmid

#68454

Purpose

TOPO construct expressing CMV for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Cytomegalovirus Immediate-Early Promoter

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pPWCh

Plasmid

#124238

Purpose

Gateway destination vector for C-terminal tagging with mCherry. The expression is driven by the UASp promoter. Suitable for Drosophila transformation and transfection.

Depositor

Peter Vilmos

Article

Vilmos et al Cell Biol Int. 2016 Jun;40(6):696-707. d

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

UASp

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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