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Plasmid#228910PurposeExpresses wildtype smooth muscle actin gamma-2 in human cellsDepositorInsertActin gamma 2, smooth muscle (ACTG2 Human)
UseTagsHIS-FLAG-TEVExpressionBacterial and MammalianMutationWTPromoterCMVAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEM-H2B-mRFP
Plasmid#196180PurposeFor in vitro transcription of H2B-mRFP mRNADepositorInsertH2B-mRFP
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPICZc-ScAct1*-thymosinB-8His
Plasmid#111148PurposeExpresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37DepositorInsertAct1 (ACT1 Budding Yeast)
UsePichia pastoris integration plasmidTagsThymosin beta and a His-tagExpressionYeastMutationPromoterAOX1Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-N1 human cofilin R21Q
Plasmid#51279Purposeexpresses human cofilin R21Q mutant fused to mRFP in mammalian cellsDepositorInsertcofilin 1 (CFL1 Human)
UseTagsmRFPExpressionMammalianMutationR21Q, non-rod-forming mutation that can be used t…PromoterCMVAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pS12<ActB.mus_2A-mCh_KI-HMEJ
Plasmid#207407PurposeDonor template to knock in mCherry at the C-terminus of mouse ActB (β-Actin) using DSB repair by homology-mediated end joining (HMEJ). [Lab plasmid ID: TU148]DepositorInsertActb (Actb Mouse)
UseCRISPR and Mouse Targeting; Hmej knockin donorTagsmCherryExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgWAVE2-2
Plasmid#210136Purposeknock out WAVE2 in mammalian cellsDepositorInsertActin-binding protein WASF2 (WASF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgWAVE2-1
Plasmid#210135Purposeknock out WAVE2 in mammalian cellsDepositorInsertActin-binding protein WASF2 (WASF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-SPN_ARR Q37A/R38A/N52R (Shank3)
Plasmid#175256PurposeSPN-ARR fragment with structure opening N52R and actin binding site Q37A/R38A mutations in EGFP backbone for mammalian expressionDepositorInsertSPN-ARR fragment with Q37A/R38A/N52R mutations from SHANK3
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMTB
Plasmid#112225PurposeExpression of membrane-tagged super folder GFP from zebrafish beta-actin promoter.DepositorInsertSuper-folder GFP
UseZebrafish expressionTagsExpressionMutationPromoterbeta actin 2 (zebrafish)Available sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::URA3
Plasmid#227595PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::URA3
UseTags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
YFP-CaaX
Plasmid#155233Purposemembane markerDepositorInsertYFP C-term tagged with the CaaX sequence derived from Kras4b. Membrane control
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-LAMP1-mGL
Plasmid#222394PurposeTo express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.DepositorInsertLAMP1 and mGreenlantern (LAMP1 Human, Synthetic, Homo Sapiens)
UseAAVTagsLAMP1 and mGreenLanternExpressionMammalianMutationPromoterCAG or CAGIGAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CFP-CaaX
Plasmid#155232Purposemembane markerDepositorInsertCFP C-term tagged with the CaaX sequence derived from Kras4b. Membrane control
UseTagsCFPExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::HIS3
Plasmid#227596PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::HIS3
UseTags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
C1-CFP-Lyn11
Plasmid#155234Purposemembane markerDepositorInsertLyn11 membrane targeted CFP. Membrane control
UseTagsCFPExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 884-1066 (aka Vt)
Plasmid#46176DepositorInsertVcl 884-1066 (VCL Chicken)
UseTagsHisExpressionBacterialMutationaa 884-1066 (tail domain)PromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::TRP1
Plasmid#227593PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::TRP1
UseTags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::LEU2
Plasmid#227594PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::LEU2
UseTags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 EGFP-3XNLS
Plasmid#58468PurposeExpresses a nuclear-targeted eGFP on a CMV promoterDepositorInsert3X NLS with 3X stop codons
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only