-
Plasmid#162615PurposeAllows for transcription of improved mVenus I152L fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assaysDepositorInsertTARDBP (TARDBP Human)
UseTagsExpressionMammalianMutationM337VPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Halo-Tev- HSPB7 (zebrafish) C49S and C117S
Plasmid#108913PurposeExpress C49S/C117S double mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cellsDepositorInsertHalo-TEV-HSPB7
UseTagsHalo-TEVExpressionMammalianMutationCysteine 49 to Serine, Cysteine 117 to SerinePromoterSP6Available sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MYC(Δ1-143)-GFP-IRES-mCherry (ΔTAD)
Plasmid#231233PurposeMYC stability reporter construct with deletion of the N-terminal region (residues 1-143) for transient expression in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsExpressionMammalianMutationTAD deleted (delta1-143); numbering based on sequ…PromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry
Plasmid#226104PurposeStability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cellsDepositorInsertCOX8A (COX8A Human)
UseTagsGFPExpressionMammalianMutationencodes only for mitochondrial targeting sequence…PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2-cDELE1(142-515)-GFP-IRES-mCherry
Plasmid#226102PurposecDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) stability reporter construct for transient expression in mammalian cellsDepositorInsertDELE1 (DELE1 Human)
UseTagsGFPExpressionMammalianMutationonly encodes for residues 142-515 of DELE1 corres…PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Myc(Δ1-143; ΔDeg1ΔDeg2)-GFP-IRES-mCherry (ΔTADΔDeg1ΔDeg2)
Plasmid#231234PurposeMYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and point mutations corresponding to the deletion of degron 1 and 2 for transient expression in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsExpressionMammalianMutationTAD deleted (Δ1-143), deletion of degron 1 & …PromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry
Plasmid#231007PurposeProtein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.DepositorInsertE4F1 aa220-242 (E4F1 Human)
UseTagsAcGFP1ExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ GFP-CDK5R1 degron (283-307)-IRES-mCherry
Plasmid#231008PurposeProtein stability reporter construct for CDK5R1 consisting of aa 283-307 for transient overexpression in mammalian cells.DepositorInsertCDK5R1 aa283-307 (CDK5R1 Human)
UseTagsAcGFP1ExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MYC(Δ1-143; Δ340-439)-GFP-IRES-mCherry (ΔTADΔDBD)
Plasmid#231235PurposeMYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and DNA-binding-domain (residues 340-439) for transient expression in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsExpressionMammalianMutationTAD deleted (delta1-143), DBD deleted (delta340-4…PromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Prom1-TEVp-TwinStrep-His / IRES2 / Pcdh21-TEVp-Myc-Flag
Plasmid#210820PurposeMammalian overexpression vector for polycistronic co-expression of C-terminally Strep and His tagged human Prom1s1 (WT) and C-terminally Myc and Flag tagged human Pchd21 (WT)DepositorUseTagsMyc, 3xFlag and TwinStrep, 10xHisExpressionMammalianMutationPromoterCMV and CMV / IRES2Available sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-NES-Myc(340-439)-GFP-IRES-mCherry (NES-DBD)
Plasmid#231237PurposeMYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NES for transient expression in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsExpressionMammalianMutationNES fused to the DNA-binding-domain of MYC (resid…PromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-NLS-Myc(340-439)-GFP-IRES-mCherry (NLS-DBD)
Plasmid#231236PurposeMYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NLS for transient expression in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsExpressionMammalianMutationNLS fused to the DNA-binding-domain of MYC (resid…PromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGCS-C3m(3xFLAG)
Plasmid#85726PurposeGateway destination vectorDepositorTypeEmpty backboneUseMammalian/avian/xenopus/zebrafishTags3xFLAGExpressionMutationPromoterAvailable sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGCS-N2(3xHA)
Plasmid#85719PurposeGateway destination vectorDepositorTypeEmpty backboneUseMammalian/avian/xenopus/zebrafishTags3xHAExpressionMutationPromoterAvailable sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGCS-N3(3xFLAG)
Plasmid#85720PurposeGateway destination vectorDepositorTypeEmpty backboneUseMammalian/avian/xenopus/zebrafishTags3xFLAGExpressionMutationPromoterAvailable sinceFeb. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGCS-C2m(3xHA)
Plasmid#85725PurposeGateway destination vectorDepositorTypeEmpty backboneUseMammalian/avian/xenopus/zebrafishTags3xHAExpressionMutationPromoterAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGCS-N1(6xMYC)
Plasmid#85718PurposeGateway destination vectorDepositorTypeEmpty backboneUseMammalian/avian/xenopus/zebrafishTags6xMYCExpressionMutationPromoterAvailable sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGCS1
Plasmid#85730PurposeGateway destination vectorDepositorTypeEmpty backboneUseMammalian/avian/xenopus/zebrafishTagsExpressionMutationPromoterAvailable sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGCS-N5(GST)
Plasmid#85722PurposeGateway destination vectorDepositorTypeEmpty backboneUseMammalian/avian/xenopus/zebrafishTagsGSTExpressionMutationPromoterAvailable sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGCS-N6(eGFP)
Plasmid#85723PurposeGateway destination vectorDepositorTypeEmpty backboneUseMammalian/avian/xenopus/zebrafishTagsEGFPExpressionMutationPromoterAvailable sinceFeb. 16, 2017AvailabilityAcademic Institutions and Nonprofits only