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T7-RelA(K310R)

Plasmid

#23250

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA (RELA Human)

Use

Tags

T7 gp10

Expression

Mammalian

Mutation

K310R

Promoter

Available since

March 15, 2010

Availability

Academic Institutions and Nonprofits only
pCW57.1-Cdx2-3xT7

Plasmid

#200891

Purpose

Inducible lentiviral expression of Cdx2

Depositor

Kai Ge

Article

Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

Insert

Cdx2 (Cdx2 Mouse)

Use

Lentiviral; Doxycycline inducible

Tags

3xT7

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_CYLD_WT

Plasmid

#82326

Purpose

Gateway Donor vector containing CYLD , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, David Root, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CYLD (CYLD Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo-Flag-DHHS

Plasmid

#85813

Purpose

To rescue the expression of DHHC5 catalytically inactive mutant in the knockdown cell lines

Depositor

Sandra Hofmann

Article

Tian et al Mol Cancer Res. 2015 Apr;13(4):784-94. d

Insert

ZDHHC5 C134S mutant (Zdhhc5 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-RIAM

Plasmid

#80030

Purpose

Mammalian expression of mCherry-RIAM (FL)

Depositor

Chinten James Lim

Insert

RIAM (APBB1IP Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pDONR223-PBK

Plasmid

#23782

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PBK (PBK Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pMy-MouseCTCFbiotag-T2A-mOrange

Plasmid

#50564

Purpose

Retroviral vector endoding for mOrange and mouse CTCF with a short biotinylation substrate (biotag, Kimetal.,2009) fused to its C-term

Depositor

Rafael Casellas

Article

Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

Inserts

Ampicilin
Mouse CTCF Exons

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pSaPIPLC His

Plasmid

#173810

Purpose

Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells

Depositor

Mary F Roberts, Anne Gershenson

Article

Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87

Insert

Staphylococcus aureus plc

Use

Tags

6XHis

Expression

Bacterial

Mutation

none

Promoter

T7

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-Padron2

Plasmid

#172355

Purpose

Expresses the reversibly switchable fluorescent protein Padron2 in bacterial cells

Depositor

Stefan Jakobs

Article

Konen et al ACS Nano. 2021 May 21. doi: 10.1021/acsn

Insert

Padron2

Use

Tags

6xHisTag (N terminal on backbone)

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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mCyRFP1-Cdc42

Plasmid

#84360

Purpose

fusion protein of Cdc42, FRET donor

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mCyRFP1-Cdc42

Use

Tags

mCyRFP1 N terminal fusion to Cdc42

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf6(WT)-HA

Plasmid

#79424

Purpose

Expresses C-terminally HA-tagged Arf6(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf6 (ARF6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pKmK.T1

Plasmid

#125053

Purpose

Storage plasmid for K.marxianus terminator; type 4 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

INU1 terminator

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-His-tag-hphMX4

Plasmid

#20764

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

Expression

Mutation

Promoter

Available since

March 27, 2009

Availability

Academic Institutions and Nonprofits only

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pMBP-HIS2-Vps2

Plasmid

#21494

Depositor

James Hurley

Article

Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

Insert

VPS2 (DID4 Budding Yeast)

Use

Tags

6x His and MBP

Expression

Bacterial

Mutation

none

Promoter

Available since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pGEX NBP1 (P#415)

Plasmid

#14658

Depositor

Matija Peterlin

Article

Lu et al Immunity. 1998 May . 8(5):647-56.

Insert

ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
GPR150-Tango

Plasmid

#66326

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR150 (GPR150 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pIREShygro HA-mVenus-mAID

Plasmid

#117714

Purpose

Expression of HA-tagged mVenus fused to mAID

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

mVenus-mAID

Use

Tags

triple HA

Expression

Mammalian

Mutation

codon optimized mAID for murine expression

Promoter

CMV

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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Kv2.1/RBG4

Plasmid

#196947

Purpose

Plasmid expressing an antigen targeting Kv2.1 (RBG4)

Depositor

James Trimmer

Insert

Kv2.1 (Kcnb1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pT7C-DNAJB6

Plasmid

#177657

Purpose

Recombinant protein synthesis of human DNAJB6

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

DNAJB6 (DNAJB6 Human)

Use

Tags

6xHis-Thrombin

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLifeAct_mScarlet-H_N1

Plasmid

#85055

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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