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We narrowed to 13,597 results for: pET

Showing: 5741 - 5760 of 13597 results

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Nsp3 Mac1 - P43 (Oxford)

Plasmid

#169210

Purpose

Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

Depositor

James S Fraser

Article

Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

Insert

SARS-CoV-2 Nsp3 Mac1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 28, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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EH1

Bacterial Strain

#102804

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC ilvA genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
biGBac Kit

Plasmid Kit

#1000000088

Purpose

Generation of baculoviral transfer vectors coding up to 25 protein complex subunits in two Gibson assembly steps. Multisubunit protein complexes can be generated in baculovirus-infected insect cells.

Depositor

Jan-Michael Peters

Available since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only
RF17

Bacterial Strain

#102801

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
RF18

Bacterial Strain

#102802

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
RF15

Bacterial Strain

#102799

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
RF16

Bacterial Strain

#102800

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB cysE genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
pGLU_115

Plasmid

#225795

Purpose

RS421 library variant 14067

Depositor

Nili Ostrov

Insert

14067_[Tn5]

Use

Synthetic Biology

Tags

Expression

Mutation

N/A

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGLU_79

Plasmid

#225780

Purpose

RS421 library variant 14067

Depositor

Nili Ostrov

Insert

14067_[abr]

Use

Synthetic Biology

Tags

Expression

Mutation

N/A

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGLU_97

Plasmid

#225810

Purpose

RS421 library variant 14067

Depositor

Nili Ostrov

Insert

14067_[Himar]

Use

Synthetic Biology

Tags

Expression

Mutation

N/A

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pEM8734

Plasmid

#213993

Purpose

PrpsM driven expression of Ypet

Depositor

Marc Erhardt

Insert

Ypet

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pJK635

Plasmid

#72473

Purpose

pET28a modified to contain TEV protease fusion tag instead of T7 tag

Depositor

T. J. Kappock

Type

Empty backbone

Use

Tags

His6 and TEV protease cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pGFP(C3)-Vinculin

Plasmid

#30312

Depositor

Klaus Hahn

Insert

Vinculin (Vcl Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

F633S, V828E, L936P in mouse viniculin (NM_009502…

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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pT7NT*-Bri2 113-231 R221E

Plasmid

#138134

Purpose

Expresseds human Bri2 BRICHOS R221E mutant in E. coli

Depositor

Jan Johansson

Article

Chen et al Commun Biol. 2020 Jan 20;3(1):32. doi: 1

Insert

human Bri2 BRICHOS R221E (ITM2B Human)

Use

Tags

NT* tag derived from spider silk proteins

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b-RFND

Plasmid

#195339

Purpose

Expresses Engineered BrCas12b in Bacteria

Depositor

Piyush Jain

Article

Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis and 6xHis, MBP, TEV cleavage site

Expression

Bacterial

Mutation

R160E, F208W, N524V, D868V

Promoter

T7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRM053 His-TEV-Cas9 triNLS 0C

Plasmid

#196244

Purpose

Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Article

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Use

CRISPR

Tags

HA, His6 (N terminal on backbone), NLS, Nucleopla…

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-pML-1B_6xHis-Cas12m

Plasmid

#192282

Purpose

MmCas12m fused to His-tag for protein purification

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmCas12m

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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WP_010880027.1

Plasmid

#194729

Purpose

Lumazine synthase of the hyperthermophile Aquifex aeolicus bacterium

Depositor

Fang Li

Article

Geng et al PLoS Pathog. 2021 Sep 7;17(9):e1009897.

Insert

nanoparticle-proteinA and His tag

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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FGFR1_HUMAN_D0

Plasmid

#79719

Purpose

This plasmid encodes the kinase domain of FGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

Markus Seeliger, John Chodera, Nicholas Levinson

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

FGFR1 (FGFR1 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pRM049 His-TEV-Cas9 triNLS 2C

Plasmid

#196245

Purpose

Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Article

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Use

CRISPR

Tags

HA, His6 (N terminal on backbone), NLS, Nucleopla…

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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