Addgene

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Showing: 5721 - 5740 of 24635 results

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pCri-4b

Plasmid

#61328

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

His-tag and TRX-tag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pa-G-GECO1.2

Plasmid

#67622

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

pa-G-GECO1.2

Use

Tags

Expression

Mammalian

Mutation

V81H, M84K, L189F, T190S

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only
pTHSSd_14

Plasmid

#59952

Purpose

Triple split (at 67, 601-SZ)

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

Three-fragment T7* RNA polymerase

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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p231 pCMVe-betaAc-STOP-luc

Plasmid

#12463

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CMVe-betaAc-STOP

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pGBW-m4133326

Plasmid

#152113

Purpose

Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

May 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Citrine-pBAD

Plasmid

#54772

Purpose

Localization: Protein Expression Vector , Excitation: 516, Emission: 529

Depositor

Roger Tsien, Oliver Griesbeck, Michael Davidson, Robert Campbell

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Use

Tags

6xHis and Citrine

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pColE1_sgRNA_pos8_AmpR

Plasmid

#119151

Purpose

Encodes sgRNA targeting position 8 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 8 in pColE1_70a_deGFP_KanR

Use

Crispr

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCM291rfp

Plasmid

#59775

Purpose

pCMrfp with TrfA mutation (291E to G)

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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myr-palm-mNeonGreen(2x)

Plasmid

#197919

Purpose

Homo-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-mNeonGreen-NeonGreen

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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myr-palm-mNeonGreen

Plasmid

#197918

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-mNeonGreen

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pTalpha1-EGFP-N1

Plasmid

#133929

Purpose

Neuron-specific expression of EGFP

Depositor

Sebastián Dupraz, Frank Bradke

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

EGFP

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046299

Plasmid

#153878

Purpose

Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-NPM NLS2D

Plasmid

#13285

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of aa150-aa155

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pCri-1b

Plasmid

#61327

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

His-tag and MBP-tag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pGR-ECK120015170

Plasmid

#46006

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

ECK120015170 Terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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enz.079-GCN5

Plasmid

#154046

Purpose

Expresses GCN5 in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

Histone acetyltransferase GCN5 (GCN5 Budding Yeast)

Use

Tags

His, MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 13, 2023

Availability

Academic Institutions and Nonprofits only

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pTCW002

Plasmid

#83522

Purpose

pheromone mediated destablised-GFP expression in yeast

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1-yEGFPCLN2PEST

Use

Tags

Expression

Yeast

Mutation

Promoter

pFUS1

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos3_AmpR

Plasmid

#119146

Purpose

Encodes sgRNA targeting position 3 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 3 in pColE1_70a_deGFP_KanR

Use

Crispr

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos2_AmpR

Plasmid

#119145

Purpose

Encodes sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 2 in pColE1_70a_deGFP_KanR

Use

Crispr

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pMLS360

Plasmid

#73687

Purpose

Punc-47::2xNLS-FLP-D5 Expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Punc-47::2xNLS-FLP-D5::let-858 3'-UTR

Use

Flp/frt

Tags

Expression

Worm

Mutation

Promoter

Punc-47

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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