Addgene

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pPOTv7-hygro-10Ty

Plasmid

#179808

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Keith Gull, Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10xTy

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pICSL80016

Plasmid

#50332

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS no stop codon, beta-glucuronidase (E. coli) with two introns

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-GFPmem no-pA

Plasmid

#80817

Purpose

GFPmem without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFPcaax no pA p3E

Use

Tags

Expression

Mammalian

Mutation

Promoter

GFP

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pSPTg.T2FXK (#54)

Plasmid

#35963

Depositor

Thomas Sato

Article

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Inserts

Tie2 promoter
Tie2 enhancer

Use

Tags

Expression

Mutation

Promoter

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pICH72400

Plasmid

#50338

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, Atug7 (A. tumefaciens)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pPRxRF3

Plasmid

#138386

Purpose

TagRFP-T knock-in cassette

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

TagRFP-T

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-1xSYN-tTA

Plasmid

#19980

Depositor

Sergey Kasparov

Article

Liu et al J Gene Med. 2008 May . 10(5):583-92.

Insert

SYN-tTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPSmOrange-SpHis5

Plasmid

#44856

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPSmOrange

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFThGW

Plasmid

#27228

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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FR_luc

Plasmid

#31098

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

firefly luciferase

Use

Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pLenti-EF1a-OCT4

Plasmid

#35391

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE-Oct4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2012

Availability

Academic Institutions and Nonprofits only
pME-mKate2 no stop

Plasmid

#80806

Purpose

red fluorophore without stop codon

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

pME-mKate2 no stop

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPS-CFP2-Kan

Plasmid

#44948

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPS-CFP2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPS-CFP2-SpHis5

Plasmid

#44853

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPS-CFP2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoLSS-mKate2-SpHis5

Plasmid

#44848

Purpose

Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoLSS-mKate2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pAGT707

Plasmid

#51835

Purpose

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Tomato Mosaic virus 5'UTR+ CCAT

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-Trex2-bpA

Plasmid

#86984

Purpose

Expression of mouse Trex2

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

mouse Trex2 coding region (Trex2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBDPR

Plasmid

#61598

Purpose

Transformation vector pBDPR

Depositor

John Carlson

Article

Koh et al Neuron. 2014 Aug 20;83(4):850-65. doi: 1

Type

Empty backbone

Use

Drosophila transformation vector

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pg50-2.11 (#65)

Plasmid

#35966

Depositor

Thomas Sato

Article

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Insert

Tie2 promoter-1st exon-1st intron

Use

Tags

Expression

Mutation

Promoter

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pICH87611

Plasmid

#50284

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Promoter and 5'UTR Ls (S.tuberosum)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge