Showing: 5661 - 5680 of 16077 results
-
Plasmid#48624PurposeExpresses Drosophila BRWD3 (with V5 and His tags at the C-terminus) in mammalian cellsDepositorInsertBRWD3 (BRWD3 Fly)
UseTagsV5 and His tagsExpressionMammalianMutationno mutations, stop was removed to fuse V5His at C…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
(114) pcDNA3.1-GFP-Flag-JunB-EPEA
Plasmid#160743PurposeExpresses a GFP tagged JunB with Flag and EPEA tags.DepositorInsertGFP-Flag-JunB-EPEA (JUN Human)
UseTagsEPEA, Flag, and GFPExpressionMammalianMutationPromoterT7/CMVAvailabilityAcademic Institutions and Nonprofits only -
pADSL-2XStrep
Plasmid#125684Purposeexpresses human ADSL protein with a C-terminal 2XStrep affinity tagDepositorInsertADSL (ADSL Human)
UseTags2X Strep-Tag IIExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-CCR5-VN
Plasmid#98963PurposeFLAG-tagged human CCR5 fused to N-terminus of split VenusDepositorInsertCCR5 (CCR5 Human)
UseTagsFLAG (dykdddd) epitope tag, Signal/leader sequenc…ExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-GRM3-VN
Plasmid#98965PurposeFLAG-tagged human mGluR3 fused to N-terminus of split VenusDepositorInsertGRM3 (GRM3 Human)
UseTagsFLAG (dykddddk) epitope tag, Signal/leader sequen…ExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pEX-PK-hLC3∆G
Plasmid#61459PurposeExpress pHluorin-mKate2-hLC3∆G (PK-hLC3∆G), a negative control for pHluorin-mKate2-hLC3 (PK-hLC3), in mammalian cells for monitoring autophagyDepositorInsertPK-hLC3∆G (MAP1LC3B Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS
Plasmid#153445PurposeE. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.DepositorInsertRc.XDocB
UseTags6xHis and ybbr tagExpressionBacterialMutationSerine 199 was mutated to amber codon (TAG). Argi…PromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AB (325-557, Δ 368-523)/pET28HMT
Plasmid#111052PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 AB (325-557, Δ 368-523)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/pET28HMT
Plasmid#111072PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Kv7.5 AB (361-545, Δ395-511)/pET28HMT
Plasmid#111267PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)
UseTagsHis Tag and MBP TagExpressionBacterialMutationKv7.5 AB (361-545, Δ395-511)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
p1A Prk-
Plasmid#162694Purposep1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)DepositorInsertH. neapolitanus CbbLS and S. elongatus Prk
UseTagsN terminal 6x His tag on PRKExpressionBacterialMutationPrk inactive (K20M S21A native protein, this map …PromoterPLtet0-1 promoterAvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-GpA-G83I
Plasmid#191239PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
UseTagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and Glycin…PromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
His-FLAG-Tev_ACTA1 (alpha-actin)
Plasmid#188452PurposeExpress in mammalian cells human alpha-actin (ACTA1) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.DepositorInsertACTA1 (ACTA1 Human)
UseTags6xHis-FLAGExpressionMammalianMutationWTPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
His-FLAG-Tev_ACTB (beta-actin)
Plasmid#188453PurposeExpress in mammalian cells human beta-actin (ACTB) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.DepositorInsertACTB (ACTB Human)
UseTags6xHis-FLAGExpressionMammalianMutationWTPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLJM1-EGFP-GRAM-H
Plasmid#211703PurposeLentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187L mutation (pLJM1-EGFP-GRAM1b G187L)DepositorInsertEGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseLentiviralTagsEGFPExpressionMammalianMutationChanged Glycine 187 to LeucinePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLJM1-EGFP-GRAM-W
Plasmid#211704PurposeLentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (pLJM1-EGFP-GRAM1b G187W)DepositorInsertEGFP-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseLentiviralTagsEGFPExpressionMammalianMutationChanged Glycine 187 to TryptophanPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pTRE-BI-osTIR1
Plasmid#207840PurposeInducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cellsDepositorInsertsosTIR
mCherry
mAID_-VHH-GFP4
UseTags3X HA-Tag and weak NLSExpressionMammalianMutationAll K mutated to RPromoterTRE3G BI promoterAvailabilityAcademic Institutions and Nonprofits only -
pET28a(cd226)
Plasmid#177900PurposeBacterial expression of his10-tagged human cd226 Intracellular DomainDepositorInsertCD226 (aa 288-336 only) (CD226 Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28a(CTLA4)
Plasmid#177905PurposeBacterial expression of his10-tagged human CTLA4 Intracellular DomainDepositorInsertCTLA4 (aa 183-223 only) (CTLA4 Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CALM1_EF-hand
Plasmid#178468PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertCALM1_EF-hand (CALM1 Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailabilityAcademic Institutions and Nonprofits only
Showing: 5661 - 5680 of 16077 results