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Plasmid#139835PurposeExpresses RNase H1-EGFP- dCas9 fusion protein in human cellsDepositorInsertRNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
UseTagsExpressionMammalianMutationdCas9 protein contains two mutations: D10A and H8…Promotertet-inducible CMVAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shCELF4-4405
Plasmid#115466PurposeConstitutive lentiviral expressionDepositorInsertshCELF4-4405 (CELF4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE3
Plasmid#90337PurposeAHR - Aryl hydrocarbon receptor gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterAHRAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFP
Plasmid#85428PurposeLentiviral vector for Gal4 inducible humanIL-12flexi with mCherry reporter and constitutive tBFP expressionDepositorInserthuman IL-12
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
SIRT5 Flag
Plasmid#13816PurposeMammalian expression of human SIRT5 with flag tagDepositorInsertSIRT5 (SIRT5 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4 human alpha5 full
Plasmid#221396PurposeMammalian expression of human integrin alpha5 full lengthDepositorInsertintegrin alpha5 full length (ITGA5 Human)
UseTagsCD33 secretion peptide (MPLLLLLPLLWAGALA) and St…ExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX458-HypaCas9
Plasmid#190604PurposePX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutationsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP13897 - pAAV-AiE0671m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3897)
Plasmid#224038PurposeAiE0671m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neuronsInsertSYFP2
UseAAVTagsExpressionMutationNAPromoterminBGAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mouse CRISPRi sgRNA library Dolomiti in backbone XPR_050
Pooled Library#1000000131PurposeGenome-wide CRISPR gRNA pooled library for inhibition of mouse genesDepositorAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Grin2b KI
Plasmid#131487PurposeEndogenous tagging of GluN2b: N-terminal (amino acid position: S34)DepositorInsertgRNA and GFP donor (Grin2b Rat)
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB_tre_dCas9_VP160
Plasmid#126031PurposePiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusionDepositorInsertsdCas9 fusion with VP160
Hygromycin resistance
UseCRISPR; PiggybacTagsExpressionMammalianMutationD10A, H840A (catalytically inactive Cas9)PromoterEF1 alpha and TREAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Human Transporter Knockout library
Pooled Library#213695PurposeNext generation transporter-focused CRISPR/Cas9 library targeting transmembrane transporters to test the impact of transporters on biological processes.DepositorSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
ALPaGA
Plasmid#175272PurposeLactate biosensor in anaerobic conditionsDepositorInsertLldR_Synthetic_promoter_sfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3
Plasmid#209730PurposeExpresses presynaptic low affinity ratiometric ATP sensorDepositorInsert(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3
UseLentiviralTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRKH2
Plasmid#71312PurposeTheta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.DepositorTypeEmpty backboneUseE. coli-gram positive bacteria shuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-FAM134B
Plasmid#109026Purposestable lentiviral expression of cDNADepositorInsertFAM134B (RETREG1 Human)
UseLentiviralTagseGFPExpressionMammalianMutationPromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-TO-hNGN2-BSD-mApple
Plasmid#124229PurposeTargets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.DepositorInsertNGN2 (NEUROG2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-hAgo2
Plasmid#21981DepositorInsertArgonaute 2 (AGO2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLEX307 RFP
Plasmid#163976PurposeMammalian expression vector encoding RFP (for example, to normalize plasmid-based protein expression)DepositorInsertRFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mito-dsRED2
Plasmid#174541Purposemitochondria targeted dsRED expressed off of EF1a promoterDepositorInsertMito
UseLentiviralTagsdsREDExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only