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Plasmid#113751PurposeGateway multi-site entry clone for second position 3x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags3xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
PcDNA-DEST53-CypB-Golgi N-term GFP
Plasmid#36127DepositorInsertcyclophilin B (PPIB Human)
UseTagsGFPExpressionMammalianMutationcontains amino acids 1-211 of cyclophilin BPromoterCMVAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFB-Flag-MyoD
Plasmid#25994DepositorInsertMyoD (Myod1 Mouse)
UseBaculovirus transfer vectorTagsFlagExpressionMutationPromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET5
Plasmid#107437PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET5 (MET5 Budding Yeast)
UseTagsV5-HisExpressionYeastMutationV81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …PromoterGAL1Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 AT1R DRY-AAY
Plasmid#45759DepositorInsertAT1R DRY-AAY (Agtr1a Rat)
UseTagsHAExpressionMammalianMutationchanged amino acids 125-126 from DR to AAPromoterCMVAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-PAmCherry1-MCS
Plasmid#60608PurposeCloning PAmCherry1DepositorInsertPAmCherry1
UseCloningTagsExpressionMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
Myog'-2A-mCherryNLS-PGK-Purodtk
Plasmid#69547PurposeDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.DepositorInsertsUseMouse TargetingTags2x nuclear localization signal and PVT1-2A "…ExpressionMutationSilent V205V mutation in myogenin gene to prevent…PromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR R4 ATCC LacZ GCTT
Plasmid#173668PurposeDestination vector for Golden Gate Cloning assemblies, blue-white screeningDepositorTypeEmpty backboneUseTagsExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
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(366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)
Plasmid#168189PurposeHA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutationDepositorInsertHA-nUbc6E-(EAAAK)4-OGT(4,K852A)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-25711gp160
Plasmid#111841PurposeMammalian expression plasmid for Env from the 25711 HIV-1 strainDepositorInsertHIV-1 (25711.2) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
ATF4 18: RLuc.5’ATF4.GFP
Plasmid#21864DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1001
Plasmid#44476DepositorInsertMinimal Mos1, unc-119, peel-1, [4-3] Gateway
UseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDisplay FLIPE-1m
Plasmid#13548DepositorInsertFLIP-E A207W
UseTagsECFP and VenusExpressionMammalianMutationA207W in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
IO201: pMVP (R4-R3) mCherry
Plasmid#121732PurposepMVP R4-R3 entry plasmid, contains mCherry for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertmCherry
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLIPE-10u
Plasmid#13542DepositorInsertFLIP-E A207R
UseTagsECFP and VenusExpressionMammalianMutationA207R in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
HGXPRT
Plasmid#64201PurposeExpression of Plasmodium falciparum HGXPRTDepositorInsertHypoxanthine guanine xanthine phosphoribosyltransferase
UseTagsHistidine tagExpressionBacterialMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-APC11
Plasmid#19898DepositorInsertAPC11 (ANAPC11 Human)
UseTagsHA and T7ExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1002
Plasmid#44477DepositorInsertMinimal Mos1, NeoR, peel-1, [4-3] Gateway
UseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceMay 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pME-QUAS5x:GFPNLS-SV40pA
Plasmid#155115PurposeMiddle entry vector containing QUAS5x element upstream of GFP-NLS.DepositorInsertQUAS5x:GFPNLS
UseGateway middle entry vectorTagsExpressionMutationPromoterQUAS5x-E1bAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only