-
Plasmid#199724PurposeA pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene. BsaI site was removed.DepositorInsertMultiple Cloning Site, NPTII
UseTagsExpressionPlantMutation"GAGACC" to "GATACC"PromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
pCMV3flag8SHARPIN
Plasmid#50014PurposeMammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.DepositorInsertSHANK-associated RH domain interactor (SHARPIN Human)
UseTags3 x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKM495 (Flag-Das+4) Zeo
Plasmid#109284PurposeORBIT integrating plasmid for C-terminal tagging with degradation tag DAS+4, using zeocin as a selection marker.DepositorInsertattB-Flag-DAS+4
UseOrbit integrating plasmidTagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-Pak1 K299R
Plasmid#12210DepositorInsertPak1 (PAK1 Human)
UseTagsMycExpressionMammalianMutationCatalytically inactive Pak1: K299RPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Plk4(Sak) wt (Nigg HR9)
Plasmid#41165DepositorInsertPlk4 (PLK4 Human)
UseTags3xMyc and PreScission SiteExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYUB1062-GFP
Plasmid#117695PurposeExpress recombinant proteins with GFP-His fusion in Mycobacterium smegmatisDepositorTypeEmpty backboneUseTagsGFP and HisExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
TALED_Right-stuffer-1397N
Plasmid#187172Purposeoriginal vector to make all constructsDepositorInsertRight-stuffer-1397N
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-N-Tandem-mCherry-EGFP
Plasmid#123216PurposeN-terminal Tandem mCherry EGFP tag. Gateway destination vector for mammalian expression.DepositorTypeEmpty backboneUseGateway destinationTagsEGFP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJ3M-Mst1 K59R
Plasmid#12204DepositorUseTagsMycExpressionMammalianMutationCatalytically inactive: K59RPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET47b-T7-dAsCas12f
Plasmid#204641PurposeExpression of an inactive AsCas12f in E. coliDepositorInsertinactive AsCas12f
UseTags6xHisExpressionBacterialMutationD225APromoterT7Available sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN6
Plasmid#180287PurposeHygR multi-gene yeast expression vector (GFP-dropout)DepositorInsertConLS' - GFP - ConRE' - HygR - CEN6/ARS4
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PKAKTRClover
Plasmid#59146PurposeORF encoding PKA KTR mClover flanked by gateway sequencesDepositorInsertPKA Kinase Translocation Reporter (PRKACA Mouse, Human)
UseTagsExpressionMutationPromoterAvailable sinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKS014
Plasmid#65467PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidaseDepositorInsertNtag-beta galactosidase
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterpl-TetOAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Myc-PSF-WT
Plasmid#35183DepositorInsertPSF (SFPQ Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRL278
Plasmid#70256PurposesacB plasmid with polylinker; kanamycin/neomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.DepositorTypeEmpty backboneUseConditionally lethalTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMVhPOP1_3Flag
Plasmid#53968PurposeMammalian expression vector for human POP1 with c-terminal 3xFLAG epitopes.DepositorInsertPOP1 (POP1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASattB-PKA-SPARK
Plasmid#106922PurposeExpress PKA-SPARK, an activity reporter of protein kinase A in DrosophilaDepositorInsertPKA-SPARK
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-SCYL1-V5-miniTurbo_IDG-K
Plasmid#189911PurposeGateway destination clone of SCYL1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertSCYL1 (SCYL1 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pY3
Plasmid#50606PurposeOrigin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5DepositorInsertstyrB
tyrA
aroC
aroA
aroL
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceNov. 14, 2014AvailabilityAcademic Institutions and Nonprofits only