Addgene

We narrowed to 11,160 results for: cel.2

Showing: 5641 - 5660 of 11160 results

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pETNKI-his-3C-LIC-Amp

Plasmid

#108704

Purpose

Expression of a His-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Use

Tags

His-3C protease cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pETNKI-strepII-3C-LIC-Amp

Plasmid

#108707

Purpose

Expression of a StrepII-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Use

Tags

StrepII-3C protease cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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Djun - antisense

Plasmid

#38338

Depositor

Norbert Perrimon

Article

Zhang et al Proc Natl Acad Sci U S A. 1990 Aug;87(16

Insert

djun (Jra Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Hsp70

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)

Plasmid

#194222

Purpose

Mammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptors

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

SynNotch-GAL4 DBD-VP64 AD

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

None

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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PGK-LCB3_v1.2-NotchGal4VP64-T2A-mCherry (pZYW047)

Plasmid

#194230

Purpose

Lentiviral expression vector expressing LCB3 SynNotch and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

PGK-LCB3-SynNotch-T2A-mCherry

Use

Lentiviral

Tags

Expression

Mutation

E45D mutation in LCB3

Promoter

PGK

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8B2(E171Q)-HA

Plasmid

#209254

Purpose

Mammalian expression of ATP8B2 mutant E171Q

Depositor

Hye-Won Shin

Article

Takatsu et al Mol Cell Biol. 2024 Sep 2:1-16. doi: 10.

Insert

ATP8B2 (ATP8B2 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

E171Q

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3- SREBP-1 (A317-318; L340)

Plasmid

#204195

Purpose

Mammalian expression of SREBP-1 (Isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Use

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317,318; Gly to Leu 340

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-1-GFP (A317-318; A341; A1171)

Plasmid

#204199

Purpose

Mammalian expression of SREBP-1 (isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Use

Tags

3xHA, eGFP and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317,318,341,1171

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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noSIIN

Plasmid

#185666

Purpose

shApc-expressing lentiviruses with no expression of SIINFEKL

Depositor

Tyler Jacks

Article

Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

Inserts

mScarlet
miR-30a
shApc

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Ef1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha2 clone2

Plasmid

#162123

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha2

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha2 (PRKAA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgAMPK alpha2 clone3

Plasmid

#162124

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha2

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha2 (PRKAA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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p7054 pHAGE-P-CMVt-N-HA-GAW-COPE

Plasmid

#100144

Purpose

Expresses COPE

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

COPE (COPE Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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tdEos-Zyxin-6

Plasmid

#57696

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Zyxin (ZYX Human)

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
CS2-Myc-dLIS1

Plasmid

#47047

Purpose

Expression of tagged Drosophila LIS1

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Lis1 (Lis-1 Fly)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only
CS2-CHY-dLIS1

Plasmid

#47045

Purpose

Expression of fluorescent Drosophila LIS1

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Lis1 (Lis-1 Fly)

Use

Tags

CHY

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-OsTIR1(F74G)

Plasmid

#140730

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-NES

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G)-P2A-mAID-EGFP-NES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)

Plasmid

#140659

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-Nluc

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G) mAID-EGFP-Nluc TOL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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HA-Elongin B-pcDNA3.1(+)-Zeo

Plasmid

#20000

Depositor

William Kaelin

Article

Ohh et al Nat Cell Biol. 2000 Jul . 2(7):423-7.

Insert

transcription elongation factor B (ELOB Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

wild type

Promoter

Available since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)

Plasmid

#140534

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G) mAID-EGFP-NLS TOL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-Tet-OsTIR1(F74G)-V5

Plasmid

#158664

Purpose

Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

Tet-OsTIR1(F74G) AAVS1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

F74G

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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