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Plasmid#148587PurposeMammalian Expression of Hs4EHP-W95AR103LE149LDepositorInsertHs4EHP-W95AR103LE149L (EIF4E2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83-V75DL79D-GB1_AB
Plasmid#148379PurposeBacterial Expression of DmThor_50-83-V75DL79DDepositorInsertDmThor_50-83-V75DL79D (Thor Fly)
UseTagsExpressionBacterialMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-H2BmGreenLantern
Plasmid#177332PurposeTo express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.DepositorInsertH2B (Hist1h2bq Rat)
UseAAVTagsmGreenLanternExpressionMammalianMutationThis fusion protein was optimized to the Human co…PromoterCAGAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW1584
Plasmid#121055PurposeYPET^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertYPET^SEC^AID*::3xFlag
UseCRISPR and Cre/LoxTags3xFLAG, C. elegans codon optimized YPET, and auxi…ExpressionWormMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPv2_FDX1-2
Plasmid#184489PurposeLentivirus all in one CRISPR vector targeting human FDX1 geneDepositorInsertFDX1 (FDX1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG 17xS/T->A, catalytic active lipin 1
Plasmid#32007DepositorInsertlipin1 (Lpin1 Mouse)
UseTagsFLAGExpressionMammalianMutationS106A, S150A, (S281A, T282A), S285A, S287A, S293A…PromoterCMV, SP6Available sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG 17xS/T->A, catalytic dead lipin 1
Plasmid#32008DepositorInsertLipin1 (Lpin1 Mouse)
UseTagsFLAGExpressionMammalianMutationD712E, D714E AND S106A, S150A, (S281A, T282A), S2…PromoterCMV, SP6Available sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPv2_FDX1-3
Plasmid#184490PurposeLentivirus all in one CRISPR vector targeting human FDX1 geneDepositorInsertFDX1 (FDX1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPv2_FDX1-1
Plasmid#184488PurposeLentivirus all in one CRISPR vector targeting human FDX1 geneDepositorInsertFDX1 (FDX1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II tension sensor (FL-based)
Plasmid#118721PurposeThe FL-based human desmoplakin II tension sensor detects forces in the range of 3-5 pN by changes in FRET between YPet(short) and mCherry.DepositorInserthuman Desmoplakin II-[YPet(short)-FL-mCherry] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherryExpressionMammalianMutationinserted FL-based tension sensor module after aa1…PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsGIGYF2_AE
Plasmid#148550PurposeMammalian Expression of HsGIGYF2DepositorInsertHsGIGYF2 (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_AE
Plasmid#148577PurposeMammalian Expression of HsGIGYF2DepositorInsertHsGIGYF2 (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II no force control (FL-based)
Plasmid#118724PurposeThe no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry] (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherryExpressionMammalianMutationtruncation after aa1353PromoterCMVAvailable sinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-MS2-HA-C1-HsGIGYF2_1-718_AI
Plasmid#148960PurposeMammalian Expression of HsGIGYF2_1-718DepositorInsertHsGIGYF2_1-718 (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_1-718_AI
Plasmid#148948PurposeMammalian Expression of HsGIGYF2_1-718DepositorInsertHsGIGYF2_1-718 (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_1-718-YFWFtoAAAA_AI
Plasmid#148950PurposeMammalian Expression of HsGIGYF2_1-718-YFWFtoAAAADepositorInsertHsGIGYF2_1-718-YFWFtoAAAA (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_280-310_AI
Plasmid#148951PurposeMammalian Expression of HsGIGYF2_280-310DepositorInsertHsGIGYF2_280-310 (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2-F300AF306A_AI
Plasmid#148952PurposeMammalian Expression of HsGIGYF2-F300AF306ADepositorInsertHsGIGYF2-F300AF306A (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2-W288A_AI
Plasmid#148953PurposeMammalian Expression of HsGIGYF2-W288ADepositorInsertHsGIGYF2-W288A (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2-W288AF300AF306A_AI
Plasmid#148954PurposeMammalian Expression of HsGIGYF2-W288AF300AF306ADepositorInsertHsGIGYF2-W288AF300AF306A (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only