Addgene

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pCDNA4-Myc-HisA-H248Y-Sirt3

Plasmid

#24917

Depositor

Toren Finkel

Article

Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

Insert

Sirt3 (SIRT3 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

His248 mutated to Tyr(Y)

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pICH51288

Plasmid

#50269

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (double), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-Purg1

Plasmid

#39349

Depositor

Jurg Bahler

Article

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

kanMX6 module - reverse orientation

Expression

Mutation

Promoter

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pFA6a-natMX6-Purg1

Plasmid

#39350

Depositor

Jurg Bahler

Article

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

natMX6 module - reverse orientation

Expression

Mutation

Promoter

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-R-Cdc42

Plasmid

#180578

Purpose

ddRFP sensor of Cdc42

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Cdc42

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2

Plasmid

#145370

Purpose

Gateway destination vector for cell-free and mamalian expression with N-term 8xHis-tag and mCherry tag

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Type

Empty backbone

Use

Tags

His and mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pICH51266

Plasmid

#50267

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (1.3 kb ), 35s (Cauliflower Mosaic Virus) + 5'UTR omega (Tobacco Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp5

Plasmid

#163589

Purpose

Expresses BMP-5 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp5 (Bmp5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

Plasmid

#86987

Purpose

For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf2

Plasmid

#163595

Purpose

Expresses GDF-2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf2 (Gdf2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCGT-Sos Cat

Plasmid

#32923

Depositor

Dafna Bar-Sagi

Article

Boykevisch et al Curr Biol. 2006 Nov 7;16(21):2173-9.

Insert

SOS1 (SOS1 Human)

Use

Tags

T7 epitope

Expression

Mammalian

Mutation

Contains aa 564-1049 (catalytic domain)

Promoter

CMV

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
L304-EGFP-Tubulin-K40R

Plasmid

#64059

Purpose

EGFP fused at the N-terminus of tubulin with a mutation K40R; lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

Insert

tubulin

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pCAG-Bmp10

Plasmid

#163593

Purpose

Expresses BMP-10 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp10 (Bmp10 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRK1037

Plasmid

#8833

Depositor

David Waugh

Article

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

TVMV protease, catalytic domain

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

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ACS2

Plasmid

#13747

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

Acetyl CoA Sythetase 2 (Acss1 Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Full-length ACS2

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry

Plasmid

#84249

Purpose

Expresses Sp sgSV40 gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgSV40

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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mSecPrP-pCB6+

Plasmid

#13917

Depositor

Susan Lindquist

Article

Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

Insert

PrP (Prnp Mouse)

Use

Tags

Expression

Mammalian

Mutation

3F4 epitag

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pPBpuro-4493NES(Booster-PKA)

Plasmid

#184709

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al Elife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pICSL70004

Plasmid

#50334

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

kanamycin/neomycin/paromomycin resistance cassette (Nos promoter (A. tumefaciens) + NptII (E.coli) + Ocs terminator (A. tumefaci

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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SpyCas9(WT)

Plasmid

#101044

Purpose

Express Streptococcus pyogenes Cas9

Depositor

Zhiwei Huang

Article

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

SpyCas9

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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