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Plasmid#131775PurposeFos upstream msfYFP 24xMS2 Fos downstream for CRISPR taggingDepositorInsertmsfYFP - 24x MS2 loops
UseCRISPRTagsExpressionMutationPromotern/aAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAS-V5-TurboID-NES
Plasmid#116904PurposeExpresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.DepositorInsertTurboID
UseDrosophila expressionTagsExpressionMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti_rtTA-P2A-BFP_HygroR
Plasmid#186738PurposertTA-P2A-BFP expression construct cloned into lentiviral backboneDepositorInsertrtTA
UseLentiviralTagsP2A-BFPExpressionMammalianMutationPromoterEF1aAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin 141-465
Plasmid#45971DepositorInsertParkin 141-465 (Prkn Rat)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Dele…PromotertacAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-Lysosomes-20
Plasmid#57793PurposeLocalization: Lysosome Membrane, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertLAMP1
UseTagsPA-mCherry-miniSOGExpressionMammalianMutationD50E in LAMP1PromoterCMVAvailable sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-Lck-GFP
Plasmid#177333PurposeExpresses GFP on the plasma membrane of neurons via an Lck localization sequenceDepositorInsertLck-GFP
UseAAVTagsGFPExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Puro-iNgn3
Plasmid#75337Purposedoxycycline inducible Ngn3 over-expressionDepositorInsertneurogenin 3 (Neurog3 Mouse)
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-Paxillin-22
Plasmid#57796PurposeLocalization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertPaxillin
UseTagsPA-mCherry-miniSOGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
LAP2 Full I pAcGFP-N1 monomeric GFP (1317)
Plasmid#62044Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertLAP2beta (Tmpo Rat)
UseTagsAcGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_CD WT TET1
Plasmid#124082PurposeExpression of wild-type catalytic domain only form of TET1DepositorInsertTET1 catalytic domain (TET1 Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterEF1 alphaAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFlox_N-Nipbl_BSD_FKBPF36V_EYFP
Plasmid#163882PurposeAcute Depletion of NIPBL-EYFPDepositorInsertNipbl (Nipbl Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_CD MUT TET1
Plasmid#124083PurposeExpression of mutated catalytic domain only form of TET1DepositorInsertTET1 - catalytic domain mutated (TET1 Human)
UseTagsFlag and HAExpressionMammalianMutationH1672Y, D1674APromoterEF1 alphaAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Puro-iNotch1IC
Plasmid#75338Purposedoxycycline inducible Notch1 intracellular domain gene over-expressionDepositorInsertNotch1 Intracellular domain (Notch1 Mouse)
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Ezr-eGFP
Plasmid#176856PurposeExpresses Ezrin fused with eGFP at the C-terminus in astrocytesDepositorInsertEzr-GFP
UseAAVTagsGFPExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI-syn-fRCaMP2
Plasmid#125246PurposeExpresses red fluorescent calcium sensor in hippocampal slicesDepositorInsertfRCaMP2
UseTagsExpressionMammalianMutationD396APromotersynapsinAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVG1
Plasmid#111444PurposeUnified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempateDepositorInsertCaCas9/sgScADE2/stop-codon repair
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-2xFYVE_hrs
Plasmid#67676PurposeMultisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrsDepositorInsert2xFYVEhrs
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
miniSOG-RatLC3
Plasmid#57775PurposeLocalization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertLC3
UseTagsminiSOGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFlox_N-p300_BSD_FKBPF36V_EYFP
Plasmid#163885PurposeAcute Depletion P300-EYFPDepositorInsertP300 (Ep300 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-fRGECO1
Plasmid#125247PurposeExpresses red fluorescent calcium sensor in mammalian cellsDepositorInsertfRGECO2
UseTagsNES signalExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only