Addgene

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SpyTag003-mClover3

Plasmid

#133453

Purpose

Expresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mClover3

Use

Tags

GSGSGSGS linker and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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UAS-Cre-T2A-miRFP670

Plasmid

#193581

Purpose

Fluorescent reporter for Gal4-induced Cre recombination

Depositor

Satoru Takahashi

Article

Yoshihara et al Exp Anim. 2022 Aug 5;71(3):385-390. doi:

Insert

miRFP670

Use

Cre/Lox

Tags

T2A

Expression

Mutation

Promoter

5X UAS

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Orai1

Plasmid

#21638

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai1 (ORAI1 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pQCascade(Ptr)-entry(BsaI)

Plasmid

#175579

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, CRISPR(BsaI)

Use

Tags

Expression

Bacterial

Mutation

NO

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pRE57I-GNAc

Plasmid

#170637

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

Mini-Tn6677, GNAc cassette, lacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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ptyrEGFP-TYR

Plasmid

#32782

Depositor

Ruth Halaban

Article

Halaban et al Proc Natl Acad Sci U S A. 2000 May 23;97

Insert

Tyrosinase (TYR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Tyrosinase

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-R-Kras

Plasmid

#180572

Purpose

ddRFP sensor of Kras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Kras

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-DIO-mCherry

Plasmid

#190241

Purpose

Expresses mCherry flanked by DIO

Depositor

Young J Yoon

Article

Nasrallah et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

mCherry

Use

AAV

Tags

N/A

Expression

Mutation

Promoter

mouse CamKII alpha

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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FR_HNF6

Plasmid

#31099

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF6 (ONECUT1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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RS314-H

Plasmid

#17541

Depositor

Bert Vogelstein

Article

Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

Insert

Gal1-p53 (TP53 Human)

Use

Tags

Expression

Yeast

Mutation

R273H

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pJCSUP35(1-253)

Plasmid

#1089

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Use

Tags

His

Expression

Bacterial

Mutation

amino acids 1 - 253 of SUP35

Promoter

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
pHAGE-EFS-dCas9-GFP

Plasmid

#64104

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

EFS

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pQFlag-USP11 WT puroR

Plasmid

#46747

Purpose

Retroviral vector that expresses Flag-tagged wild type human USP11

Depositor

Gordon Peters, Goedele Maertens

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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FUW-SO

Plasmid

#20329

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-F2A-Oct4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-hPROX1-tdTomato-PuroTK

Plasmid

#141027

Purpose

Reporter integration

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pV132-vCas9

Plasmid

#203223

Purpose

Mammalian expression of vCas9

Depositor

Robert Langer, Phil Sharp, Vikash Chauhan

Article

Chauhan et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

vCas9

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

S55R R976A K1003A T1314R

Promoter

CBh

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W

Plasmid

#112927

Purpose

CRISPR-a reporter assay lentiviral vector (gTetO)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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hTRIM65_EGFP_N1

Plasmid

#54320

Purpose

Mammalian expression vector for human TRIM65 fusion protein with c-terminal EGFP.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

TRIM65 (TRIM65 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA-myc-HisA-Sirt3

Plasmid

#24924

Depositor

Toren Finkel

Article

Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

Insert

Sirt3 (SIRT3 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-G-Hras

Plasmid

#180573

Purpose

ddGFP sensor of Hras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Hras

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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