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Plasmid#131808PurposeVector with aTc inducible M. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 25 and 26 with a C-terminal fused mRFP1DepositorArticleInsertM. laminosus Fd1 mutant with the estrogen recepor-ligand binding domain fused between amino acids 25 and 26
UseSynthetic BiologyTagsmRFP1ExpressionMutationPromoterAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD_LSSmOrange
Plasmid#118860Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertLSSmOrange
UseTagsHis-tagExpressionBacterialMutationA44V,F84L, W145M, I165D, M167L, G202D substituti…PromoterAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19_extendedTTE1564
Plasmid#60999PurposeT7-transcription template for Tte mRNA used in SiM-KARTSDepositorInsertTTE1564 transcript
UseUnspecifiedTagsExpressionMutationPromoterT7Available sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMZ039
Plasmid#67757PurposeExpresses N-terminal kinase domain of Chk1 with C-term GFP fluorescent tagDepositorInsertzebrafish Chk1
UseTagsGFPExpressionMutationonly encodes N-term kinase domain (amino acids 1-…PromoterAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMPO 51
Plasmid#68358PurposeExpression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2). ApR, expression vector with rrnBT1T2-Pm-MCSII, ColE1 replication originDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET28-p53 (Guinea Pig 1-88)
Plasmid#62059PurposeExpression of Guinea Pig p53 (residues 1-88) in e. coliDepositorInsertp53
UseTagsHisExpressionBacterialMutationcontains TAD residues 1-88PromoterT7Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pattB-synaptobrevin-13-QFBDAD-G4MD257-761-hsp70
Plasmid#46122DepositorInsertQFBDAD-G4MD257-761
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pattB-synaptobrevin-12-QFBDAD-G4MD50-761-hsp70
Plasmid#46121DepositorInsertQFBDAD-G4MD50-761
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQC_HA-p115 delta C76 #90#410
Plasmid#42129DepositorInsertp115
UseRetroviralTagsHAExpressionMammalianMutationdeleted C-terminal 76 aaPromoterAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
XE51 XPKC-alpha
Plasmid#16746DepositorInsertXPKC-Alpha (prkca Frog)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKB236
Plasmid#170027PurposeEncodes LacI-controlled expression of surface-displayed TCP and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
TCP
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Floxed-STOP mCherry
Plasmid#122963PurposemCherry-based fluorescent reporter for Cre-loxP DNA recombination reactionDepositorInsertpcDNA3.1_Floxed-STOP mCherry
UseCre/LoxTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN4
Plasmid#180285PurposeKanR multi-gene yeast expression vector (GFP-dropout)DepositorInsertConLS' - GFP - ConRE' - KanR - CEN6/ARS4
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2(+)-zGrad
Plasmid#119716PurposePlasmid to generate zGrad mRNA to inactivate proteins in zebrafishDepositorInsertzGrad
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVpuro-CMV-N-3xFLAG
Plasmid#123223PurposeN-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.DepositorTypeEmpty backboneUseLentiviral; Gateway destinationTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVpuro-CMV-N-EGFP
Plasmid#122848PurposeN-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.DepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVpuro-CMV-N-mCherry
Plasmid#123221PurposeN-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.DepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8.20
Plasmid#194702PurposeExpresses N-terminal MBP fused TadA8.20 in bacteriaDepositorInsertTadA8.20
UseTags6xHis and MBPExpressionBacterialMutationmutations in TadA are described in the paperPromoterT7 promoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHEA
Plasmid#168297PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
UseTags6xHis, E-tag, and PelB signal peptideExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only