-
-
rag1 short
Plasmid#11300DepositorInsertrag1 (rag1 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:GFP-CAAX
Plasmid#184813PurposeUsed to generate the Tg(QUAS:GFP-CAAX)c591 transgenic lineDepositorArticleInsertQUAS:GFP-CAAX-pA
UseZebrafish tol2 transgenesisTagsGFP-CAAXExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-B3GALT5
Plasmid#192719PurposeGateway entry vector encoding human B3GALT5DepositorInsertB3GALT5 (B3GALT5 Human)
UseGateway entry vectorTagsExpressionMutationM85TPromoterNoneAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol2-UAS-zArchon1-KGC-EGFP-ER2
Plasmid#108427PurposeCodon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmidDepositorInsertzArchon1-KGC-EGFP-ER2
UseZebrafishTagsExpressionMutationPromoterUASAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135766PurposeThis plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorInsertWilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)
UseZebrafishTagsExpressionMutationPromoterUASAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol2-UAS-zArchon2-KGC-EGFP-ER2
Plasmid#108428PurposeCodon-optimized Archon2 fluorescent voltage reporter in zebrafish expression plasmidDepositorInsertzArchon2-KGC-EGFP-ER2
UseZebrafishTagsExpressionMutationPromoterUASAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSYC-204
Plasmid#178964PurposeHuman GFAP (R79C) mutant fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombinationDepositorInsertsUseTol2 mediated recombinationTags3xFLAGExpressionMutationR79CPromoterZebrafish gfap promoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSYC-201
Plasmid#178963PurposeHuman GFAP fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombinationDepositorInsertsUseTol2 mediated recombinationTags3xFLAGExpressionMutationPromoterzebrafish gfap promoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2 Gateway compatible toolbox for the study of the nervous system and neurodegenerative disease
Plasmid Kit#1000000087PurposeTol2 Zebrafish toolkit: 4 promoters, 6 fluorophores, MultiSite Gateway (Invitrogen) compatible and cloning sites. Production transgenic zebrafish for neurodegenerative disordersDepositorAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Arl13b-mCardinal
Plasmid#194542PurposeExpresses Arl13b with mCardinal in mammalian cells and zebrafish embryosDepositorInsertArl13b (Arl13b Mouse)
UseTagsmCardinalExpressionMammalianMutationPromoterCMV IE94Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5E-elavl3
Plasmid#75025PurposeCan be used to drive pan neuronal expression in zebrafish.DepositorInsertELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
Use5' entry vector for multisite gateway three …TagsExpressionMutationPromoterelavl3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5E-gfap
Plasmid#75024PurposeCan be used to drive expression in zebrafish astrocytes.DepositorInsertglial fibrillary acidic protein promoter (gfap Zebrafish)
Use5' entry vector for multisite gateway three …TagsExpressionMutationPromotergfapAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-sox10-NLS-BirA-2A-mCherry_Ras
Plasmid#80063PurposeSox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterSox10 promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT3TS nErCas12an
Plasmid#132642PurposeUsed for in vitro transcription of dual NLS ErCas12a mRNADepositorInsertErCas12a
UseCRISPR; In vitro transcriptionTagsSV40 NLSExpressionMutationPromoterT3Available sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
ins:GCaMP6s; cryaa:RFP
Plasmid#110285PurposeA zebrafish insulin promoter driving the calcium indicator, GCaMP6s. Contains red eye marker, and is flanked with I-SceI sites.DepositorInsertGCaMP6s
UseZebrafishTagsExpressionMutationPromoterinsulinAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnk9-IRES-EGFP
Plasmid#164949PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnk9 and EGFPDepositorInsertkcnk9 (kcnk9 Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoteractinb (actb2) promoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS
Plasmid#78251PurposeCan be used to subclone in tagged gene of interest into inducible motor neuron expression vector.DepositorInsertsUseZebrafish motor neuron inducible expressionTagsExpressionMutationPromoter-3mnx1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2- mRuby-Rab35
Plasmid#194338PurposeExpresses mRuby Rab35 in mammalian cells and zebrafish embryosDepositorInsertRab35 (RAB35 Human)
UseTagsmRubyExpressionMammalianMutationPromoterCMV IE94Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT-sox10-cytoBirA-2A-mCherry_Ras
Plasmid#80062PurposeSox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterSox10 promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only
