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Plasmid#35632DepositorInsertN-Ras(G12V) (NRAS Human)
UseLentiviralTagsHAExpressionMammalianMutationGlycine 12 to ValinePromoterPGKAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRPF215
Plasmid#106377PurposeC. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposonDepositorInsertPtet-himar1-Ter(slpA)-transposon
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationCodon optimised for C. difficilePromoterPtetAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
p12x601
Plasmid#157785PurposeEcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backboneDepositorInsertpMD2.G(spei/spei)&12x601_tetO
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_30bpLinker
Plasmid#157789PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_30bp linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTF102
Plasmid#134774PurposeA binary plasmid for plant transformation containing _-glucuronidase gene driven by CaMV 35S promoter and a bialaphos resistance gene (bar) driven by CaMV 35S promoter and TEV enhancer.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIS3
Plasmid#92173PurposeVector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by disruption of the region targeted for recombination.DepositorInsertNAT1
UseGenbank: u03440.1TagsExpressionYeastMutationPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMARO
Plasmid#178086PurposeDerivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.DepositorInsertTcR (tetA/tetR)
UseTagsExpressionBacterialMutationTetracycline resistance cassette is flanked by Ec…PromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
hCas9-VPR
Plasmid#68497Purposenuclease competent SP-Cas9 fused to VPRDepositorInsertSP-Cas9-VPR
UseTagsVPRExpressionMammalianMutationPromoterCMVAvailable sinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHBintT
Plasmid#48134PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
mcherry-Myo 9b
Plasmid#134918PurposeExpression of rat Myo 9b (Myr 5) in mammalian cells.DepositorInsertMyo 9b (rat) with 3' UTR (Myo9b Rat)
UseTagsmcherryExpressionMammalianMutationPromoterCMV IEAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
3XFlag pCMV5-TOPO TAZ WT
Plasmid#24809DepositorInsertTAZ (WWTR1 Human)
UseTags3XFlagExpressionMammalianMutationPromoterAvailable sinceMay 14, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHRdSV40_scFv_GCN4_sfGFP-VPR-GB1_NLS
Plasmid#79373PurposeRecruits VPR to a compatible Cas9 protein for transcriptional activationDepositorInsertGCN4-sfGFP-VPR
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9
Plasmid#78903PurposeExpresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cellsDepositorInsertdCas9
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_193 NRL + TetO
Plasmid#157812PurposeEcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_tetO
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_45bpLinker
Plasmid#157791PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_45bp linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/CyclinB1-GFP (28)
Plasmid#39871DepositorInsertCyclinB1 (CCNB1 Human)
UseTagsGFPExpressionMammalianMutationV5APromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTP443
Plasmid#216361PurposexNup98-Nb1t (+1Cys) Nanobody productionDepositorInsertxNup98-Nb1t (+1Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBintN
Plasmid#48135PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker neomycine for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHBintE
Plasmid#48136PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
gRNA_tdtomato_reporter1_SP-2x
Plasmid#79370PurposeAssays activity of transcriptional activators fused to SP Cas9DepositorInserttdtomato
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2017AvailabilityAcademic Institutions and Nonprofits only